评价新型自动化 Atellica Hema 580 血液分析仪的性能。

Performance evaluation the new automated Atellica Hema 580 hematology analyzer.

机构信息

Department of Molecular Medicine and Hematology, University of Witwatersrand Medical School, Johannesburg, South Africa.

Department of Molecular Medicine and Hematology, National Health Laboratory Service, Johannesburg, South Africa.

出版信息

Int J Lab Hematol. 2024 Feb;46(1):63-71. doi: 10.1111/ijlh.14170. Epub 2023 Sep 20.

DOI:10.1111/ijlh.14170

PMID:37731006

Abstract

INTRODUCTION

The Atellica Hema (Siemens Healthineers, Tarrytown, NY, USA) is a new generation multi-parameter analyzer for full blood count, 6-part differential and reticulocyte testing by impedance variation and fluorescence flow cytometry. In this study, we verified the whole blood and limited body fluid modes of the Atellica Hema 580.

METHODS

We evaluated precision, linearity, carry-over, throughput and performed a method comparison to assess the performance of the Atellica Hema 580. For comparison of the Atellica Hema 580 with the Sysmex XN-1000 (Sysmex, Kobe, Japan), 140 samples from adult and pediatric patients including both normal and abnormal hematology profiles were analyzed in parallel.

RESULTS

The Atellica Hema 580 demonstrated acceptable imprecision within the manufacturer's specifications for whole blood and body fluid modes, good linearity for high and low ranges and no significant carryover. The full blood count, differential and reticulocyte correlated well with the Sysmex XN-1000, except for mean cell hemoglobin concentration, basophil and large immature cells. The optical platelet count, reflexed in 34 samples with a platelet count <150 × 10 /l, showed a strong correlation with the fluorescent platelet count on the Sysmex XN-1000. The morphology flagging efficiency was 92% for white blood cells, 95% for red blood cells and 87% for platelets.

CONCLUSION

The Atellica Hema 580 showed good analytical performance and workflow efficiency for a wide range of patient samples.

摘要

简介

Atellica Hema（西门子健康，纽约州塔里敦，美国）是新一代的多参数分析仪，用于全血细胞计数、6 部分差异和网织红细胞检测，采用阻抗变化和荧光流动 cytometry。在这项研究中，我们验证了 Atellica Hema 580 的全血和有限体液模式。

方法

我们评估了精密度、线性、携带、吞吐量，并进行了方法比较，以评估 Atellica Hema 580 的性能。为了比较 Atellica Hema 580 与 Sysmex XN-1000（Sysmex，神户，日本），我们对包括正常和异常血液学特征的成人和儿科患者的 140 个样本进行了平行分析。

结果

Atellica Hema 580 在全血和体液模式下的制造商规格内表现出可接受的精密度，高和低范围的良好线性，没有显著的携带。全血细胞计数、差异和网织红细胞与 Sysmex XN-1000 相关性良好，除了平均细胞血红蛋白浓度、嗜碱性粒细胞和大不成熟细胞外。光学血小板计数，在 34 个血小板计数<150×10 /l 的样本中反射，与 Sysmex XN-1000 上的荧光血小板计数有很强的相关性。白细胞的形态标记效率为 92%，红细胞为 95%，血小板为 87%。