Tanaka Yuzo, Tanaka Yumiko, Gondo Kazumi, Maruki Yoshiko, Kondo Tamiaki, Asai Satomi, Matsushita Hiromichi, Miyachi Hayato
Clinical Laboratory, Tokai University Hospital, Isehara, Japan.
J Clin Lab Anal. 2014 Sep;28(5):341-8. doi: 10.1002/jcla.21691. Epub 2014 Mar 19.
Conventional automated hematology analyzers have limitations in platelet measurements such as poor accuracy and precision in the low count range and interference by nonplatelet particles. In order to improve it, the newly developed XN-Series automated hematology analyzers (Sysmex Corporation, Kobe, Japan) have been installed with a new dedicated channel for platelet analysis (PLT-F), which is based on a fluorescence flow cytometry method with uses of a novel fluorescent dye specifically staining platelets. We evaluated the basic performance of this new PLT-F channel.
Basic performance of the PLT-F channel in within-run reproducibility and assay linearity was studied using standard methods. Correlation was studied between PLT-F and a conventional automated hematology analyzer (XE-2100) and immunoplatelet analysis using anti-CD61 monoclonal antibody (Cell-Dyn Sapphire; Abbott Laboratories). The assay interference by nonplatelet particles such as fragmented red and white blood cells was evaluated by using clinical samples, respectively, from burn injury and acute leukemia.
Basic performance of the PLT-F platelet counting was satisfactory in within-run reproducibility, linearity and correlation with the conventional analyzer. The correlation was satisfactory also with the immunoplatelet analysis, even for samples from a patient with burn injury, and those with white blood cell fragments displayed, platelet abnormal flag and low platelet counts (<50 × 10(9)/l).
The platelet counting performance of the PLT-F channel of the XN Series had improved accuracy and precision in the low range and in abnormal samples, avoiding the interference by nonplatelet particles.
传统的自动血液分析仪在血小板检测方面存在局限性，如在低计数范围内准确性和精密度较差，以及受到非血小板颗粒的干扰。为了改进这一情况，新开发的XN系列自动血液分析仪（日本神户Sysmex公司）安装了一个用于血小板分析的新专用通道（PLT-F），该通道基于荧光流式细胞术方法，使用一种专门对血小板进行染色的新型荧光染料。我们评估了这个新PLT-F通道的基本性能。
使用标准方法研究PLT-F通道在批内重复性和分析线性方面的基本性能。研究了PLT-F与传统自动血液分析仪（XE-2100）以及使用抗CD61单克隆抗体的免疫血小板分析（Cell-Dyn Sapphire；雅培实验室）之间的相关性。分别使用烧伤和急性白血病患者的临床样本评估非血小板颗粒（如破碎的红细胞和白细胞）的分析干扰。
PLT-F血小板计数在批内重复性、线性以及与传统分析仪的相关性方面基本性能令人满意。与免疫血小板分析的相关性也令人满意，即使对于烧伤患者的样本以及那些有白细胞碎片、显示血小板异常标记和低血小板计数（<50×10⁹/L）的样本也是如此。
XN系列PLT-F通道的血小板计数性能在低范围和异常样本中具有更高的准确性和精密度，避免了非血小板颗粒的干扰。
