Guangdong Provincial Key Laboratory of Advanced Biomaterials, Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, China.
Shenzhen Key Laboratory of Pathogen and Immunity, National Clinical Research Center for Infectious Disease, State Key Discipline of Infectious Disease, Infectious Disease Department, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology, Shenzhen, China.
Nat Biomed Eng. 2023 Dec;7(12):1636-1648. doi: 10.1038/s41551-023-01092-4. Epub 2023 Sep 21.
Portable assays for the rapid identification of lineages of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to aid large-scale efforts in monitoring the evolution of the virus. Here we report a multiplexed assay in a microarray format for the detection, via isothermal amplification and plasmonic-gold-enhanced near-infrared fluorescence, of variants of SARS-CoV-2. The assay, which has single-nucleotide specificity for variant discrimination, single-RNA-copy sensitivity and does not require RNA extraction, discriminated 12 lineages of SARS-CoV-2 (in three mutational hotspots of the Spike protein) and detected the virus in nasopharyngeal swabs from 1,034 individuals at 98.8% sensitivity and 100% specificity, with 97.6% concordance with genome sequencing in variant discrimination. We also report a compact, portable and fully automated device integrating the entire swab-to-result workflow and amenable to the point-of-care detection of SARS-CoV-2 variants. Portable, rapid, accurate and multiplexed assays for the detection of SARS-CoV-2 variants and lineages may facilitate variant-surveillance efforts.
需要便携式检测方法来快速鉴定严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的谱系，以协助大规模监测病毒的演变。在此，我们报告了一种基于微阵列的多重检测方法，通过等温扩增和等离子体金增强近红外荧光检测 SARS-CoV-2 的变体。该检测方法具有针对变体区分的单核苷酸特异性、单个 RNA 拷贝的灵敏度，并且不需要 RNA 提取，可区分 SARS-CoV-2 的 12 个谱系（在 Spike 蛋白的三个突变热点中），并在 1,034 名个体的鼻咽拭子中以 98.8%的灵敏度和 100%的特异性进行检测，在变体鉴别方面与基因组测序的符合率为 97.6%。我们还报告了一种紧凑、便携且完全自动化的设备，集成了整个拭子到结果的工作流程，适用于 SARS-CoV-2 变体的即时检测。用于检测 SARS-CoV-2 变体和谱系的便携式、快速、准确和多重检测方法可能有助于变体监测工作。
