Shevitz J, Jenkins C S, Hatcher V B
Mech Ageing Dev. 1986 Aug;35(3):221-32. doi: 10.1016/0047-6374(86)90125-9.
Fibronectin was measured in early and late passaged human skin fibroblasts utilizing immunoprecipitation and polyacrylamide gel electrophoresis. A progressive increase in the rate of fibronectin and total cellular protein synthesis per cell was observed by late passaged human diploid fibroblasts. The absolute protein concentration increased in the late passaged fibroblasts. There was no significant difference in the [3H]leucine incorporation into fibronectin or total cellular protein/mg protein. The turnover of fibronectin and total cellular protein did not differ between early and late passaged fibroblasts. The transport of fibronectin to the cell membrane was similar in late passaged fibroblasts. The increased fibronectin synthesis in senescent fibroblasts appeared to correlate with the general increase in rate of protein synthesis/cell.
利用免疫沉淀和聚丙烯酰胺凝胶电泳法对早期传代和晚期传代的人皮肤成纤维细胞中的纤连蛋白进行了测定。晚期传代的人二倍体成纤维细胞显示，每个细胞中纤连蛋白和总细胞蛋白的合成速率逐渐增加。晚期传代的成纤维细胞中绝对蛋白浓度升高。在纤连蛋白或总细胞蛋白/毫克蛋白中，[3H]亮氨酸掺入量没有显著差异。早期传代和晚期传代的成纤维细胞之间，纤连蛋白和总细胞蛋白的周转率没有差异。晚期传代的成纤维细胞中，纤连蛋白向细胞膜的转运情况相似。衰老成纤维细胞中纤连蛋白合成增加似乎与每个细胞蛋白质合成速率的总体增加相关。
