Fodil-Bourahla I, Drubaix I, Robert L, Labat-Robert J
Laboratoire d'Immunologie du Vieillissement, Equipe de Biochimie du Tissu Conjonctif, Service d'ophtalmologie Hôtel-Dieu, Paris, France.
Gerontology. 1999 Jan-Feb;45(1):23-30. doi: 10.1159/000022051.
The 67-kD elastin-laminin receptor (ELR) subunit which carries the recognition site for elastin peptides (EP) is a lectin. Its binding with galactosides can modulate the kinetics of its interaction with its ligand, EP. In this study the biosynthesis of proteins, collagen and fibronectin were evaluated in the presence of agonists and antagonists of the receptor on human skin fibroblasts. The biosynthesis of total proteins determined by 3H-proline incorporation and of fibronectin (by immunoprecipitation) were shown to increase with passage number. The presence of 1 microg/ml kappa-elastin (EP) in the culture medium increased both total proteins and fibronectin biosynthesis. Melibiose, an agonist of the receptor at 5 microg/ml (140 microM), decreased both proteins and fibronectin biosynthesis in the culture medium of human skin fibroblast at the 10th and 15th passage. These results show that the ELR can control the biosynthetic mechanisms of some of the macromolecular constituents of extracellular matrix such as fibronectin and moderate its age and passage-dependent upregulation.
携带弹性蛋白肽（EP）识别位点的67-kD弹性蛋白-层粘连蛋白受体（ELR）亚基是一种凝集素。它与半乳糖苷的结合可调节其与配体EP相互作用的动力学。在本研究中，在人皮肤成纤维细胞上存在受体激动剂和拮抗剂的情况下，评估了蛋白质、胶原蛋白和纤连蛋白的生物合成。通过3H-脯氨酸掺入法测定的总蛋白质生物合成以及通过免疫沉淀法测定的纤连蛋白生物合成均显示随着传代次数增加而增加。培养基中1微克/毫升κ-弹性蛋白（EP）的存在增加了总蛋白质和纤连蛋白的生物合成。蜜二糖，一种浓度为5微克/毫升（140微摩尔）的受体激动剂，在第10代和第15代人皮肤成纤维细胞的培养基中降低了蛋白质和纤连蛋白的生物合成。这些结果表明，ELR可以控制细胞外基质的一些大分子成分（如纤连蛋白）的生物合成机制，并调节其随年龄和传代的上调。
