Sandor P, Cox-van Put J, de Jong W, de Wied D
Life Sci. 1986 Nov 3;39(18):1657-65. doi: 10.1016/0024-3205(86)90163-3.
The validity of a photoelectric method for continuous cerebral blood volume (CBV) measurement was tested and modified for the rat's brain. A new way of introducing a miniature light source between the two hemispheres and fixing a light sensitive silicone blue cell to the outer surface of the parietal bone was developed. Light extinction factor of the rat's blood was determined experimentally (Eb rat = 1.38 +/- 0.15) in order to calculate absolute CBV value in this species, resulting in a 4.77 +/- 0.13 vol % absolute CBV value. Data obtained in anesthetized, artificially ventilated rats by simultaneous recording of CBV and local cerebral blood flow (H2-gas clearance technique) show that local hypothalamic blood flow decreased significantly after morphine (1.0 mg/kg s.c.), while total CBV remained unchanged. Opiate receptor blockade with naloxone (1.0 mg/kg s.c.) on the contrary, as well as naloxone and morphine administration, caused no change in local hypothalamic blood flow, but resulted in a significant increase of total cerebral blood volume.
对一种用于连续测量脑血容量（CBV）的光电方法在大鼠脑内的有效性进行了测试和改进。开发了一种在两个半球之间引入微型光源并将光敏硅蓝细胞固定在顶骨外表面的新方法。通过实验确定了大鼠血液的光消光系数（Eb大鼠 = 1.38 +/- 0.15），以便计算该物种的绝对CBV值，得出绝对CBV值为4.77 +/- 0.13体积%。在麻醉、人工通气的大鼠中，通过同时记录CBV和局部脑血流（H2气体清除技术）获得的数据表明，吗啡（1.0 mg/kg皮下注射）后下丘脑局部血流显著减少，而总CBV保持不变。相反，用纳洛酮（1.0 mg/kg皮下注射）进行阿片受体阻断，以及纳洛酮和吗啡给药，均未引起下丘脑局部血流变化，但导致全脑血容量显著增加。
