Tomita M, Gotoh F, Sato T, Amano T, Tanahashi N, Tanaka K, Yamamoto M
Am J Physiol. 1978 Jul;235(1):H56-63. doi: 10.1152/ajpheart.1978.235.1.H56.
A new photoelectric method for measuring hemodynamic changes in a thin layer of cerebral tissue in situ is described. The apparatus consists of a miniature lamp of 0.7 mm OD and a photodiode with a color filter having maximum light transmission at 548 nm. The lamp was chronically or acutely implanted into the brain tissue of animals as a light source, and the intensity of the light transmitted through the cerebral tissue was monitored with the photodiode from the brain surface. The intensity of the transmitted light was quantified for cerebral blood volume (CBV). The mean transit time of blood (t) was obtained from tissue hemodilution curves produced by saline injection into the carotid artery. The regional blood flow was calculated from the values of CBV and t according to the Stewart-Hamilton equation. The apparatus was shown to be stable, rapid in response, easy to operate, and to give reproducible results. Possible sources of error are discussed.
本文描述了一种用于原位测量薄层脑组织血流动力学变化的新型光电方法。该装置由外径为0.7毫米的微型灯和带有在548纳米处具有最大透光率的滤色片的光电二极管组成。该灯作为光源被长期或急性植入动物脑组织中，透过脑组织的光强度由光电二极管从脑表面进行监测。透过光的强度被量化以测量脑血容量（CBV）。血液的平均通过时间（t）由向颈动脉注射生理盐水所产生的组织血液稀释曲线获得。根据斯图尔特 - 哈密顿方程，由CBV和t的值计算局部血流量。结果表明该装置稳定、响应迅速、操作简便且结果可重复。文中还讨论了可能的误差来源。
