Thammasat University Research Unit in Smart Materials and Innovative Technology for Pharmaceutical Applications (SMIT-Pharm), Faculty of Pharmacy, Thammasat University, Pathumthani 12120, Thailand.
College of Pharmacy, Rangsit University, Pathumthani 12000, Thailand.
Carbohydr Polym. 2023 Dec 1;321:121294. doi: 10.1016/j.carbpol.2023.121294. Epub 2023 Aug 12.
A pullulan (Pul)-derivative hydrogel was developed by introducing methacrylate (MA) groups and β-cyclodextrin (βCD) to form a Pul-βCD-MA hydrogel by UV cross-linking. The MA was expected to improve the hydrogel's mechanical properties and the βCD to increase the solubility of curcumin. Pul-βCD-MA was successfully synthesized, as confirmed by the H NMR and FTIR spectra. Hydrogels were formed at Pul-βCD-MA concentrations of 5 %, 7.5 %, or 10 % w/v. Pul-βCD-MA showed enhanced curcumin solubility compared to Pul or Pul-MA. The morphological analysis of the hydrogel showed a porous structure. The concentration of βCD affected the hydrogels' mechanical properties, and the 7.5 % hydrogel (with or without curcumin) did not fracture. The cumulative release of curcumin in the 7.5 % Pul-βCD-MA hydrogel was 60 % over 8 h. The release profile fit the Korsmeyer-Peppas model. In vitro cytotoxicity tests revealed that hydrogels were biocompatible with human primary dermal fibroblast cells. Curcumin-loaded Pul-βCD-MA hydrogels significantly accelerated wound healing compared to Pul-βCD-MA hydrogels without curcumin loading. Therefore, the Pul derivative's hydrogel may be a promising material for wound healing.
一种普鲁兰（Pul）衍生水凝胶是通过引入甲基丙烯酰基（MA）基团和β-环糊精（βCD），通过 UV 交联形成 Pul-βCD-MA 水凝胶而开发的。预计 MA 可以改善水凝胶的机械性能，βCD 可以增加姜黄素的溶解度。成功合成了 Pul-βCD-MA，通过 1H NMR 和 FTIR 光谱证实了这一点。在 5%、7.5%或 10%w/v 的 Pul-βCD-MA 浓度下形成水凝胶。与 Pul 或 Pul-MA 相比，Pul-βCD-MA 显示出增强的姜黄素溶解度。水凝胶的形态分析显示出多孔结构。βCD 的浓度影响水凝胶的机械性能，而没有姜黄素的 7.5%水凝胶（有或没有姜黄素）没有断裂。在 8 小时内，7.5%Pul-βCD-MA 水凝胶中姜黄素的累积释放量为 60%。释放曲线符合 Korsmeyer-Peppas 模型。体外细胞毒性试验表明，水凝胶与人原代真皮成纤维细胞相容。与不含姜黄素负载的 Pul-βCD-MA 水凝胶相比，负载姜黄素的 Pul-βCD-MA 水凝胶显著加速了伤口愈合。因此，Pul 衍生物的水凝胶可能是一种有前途的伤口愈合材料。
