Department of Engineering Sciences, Middle East Technical University, Ankara 06800, Turkey; BIOMATEN, CoE in Biomaterials & Tissue Engineering, Middle East Technical University, Ankara 06800, Turkey.
Department of Molecular Biology and Genetics, Istanbul Technical University, Istanbul 34758, Turkey.
Int J Biol Macromol. 2022 Oct 1;218:760-774. doi: 10.1016/j.ijbiomac.2022.07.160. Epub 2022 Jul 25.
Vitamin C&E (VtC&VtE)-loaded bilayer wound dressings were prepared using bacterial cellulose (BC) synthesized by Acetobacter species and pullulan (PUL). VtC-containing PUL hydrogels (100 μg/mL) were immobilized onto BC by crosslinking. BC/PUL-VtC was loaded with VtE (100 μM in ethanol) by immersion for 2 h. No delamination between the layers was observed via SEM. Despite the porous inner PUL side, the outer BC side exhibited nanofibrous morphology serving as barriers to prevent microorganism invasion. Equilibrium water content of BC/PUL was above 85 % due to the hydrogel characteristics of PUL side, suitable to absorb exudate in wound bed. PUL layer lost >90 % of its weight in simulated wound fluid and > 99 % in lysozyme solution within 14 days, mediating co-release of VtC&VtE. Thin BC side possessed adequate strength (⁓22 MPa) and strain (>30 %) to endure against tensile stress generated by bending on wound surface without rupture, whereas thick PUL side was flexible (>70 % strain) to fit into wound bed under compressive stress without causing harm. In vitro studies using L929 fibroblasts elucidated PUL side was anti-adhesive and removable. Synergistic effect of VtC&VtE on antioxidant activity, wound closure, and collagen synthesis was observed. Thus, BC/PUL-VtC/VtE hold promise as cheap and eco-friendly temporary wound dressing.
载有维生素 C 和 E（VtC 和 VtE）的双层伤口敷料由醋酸菌合成的细菌纤维素（BC）和普鲁兰（PUL）制备。将含有 VtC 的 PUL 水凝胶（100μg/mL）通过交联固定在 BC 上。BC/PUL-VtC 通过浸渍 2 小时负载 VtE（乙醇中的 100μM）。通过 SEM 观察到各层之间没有分层。尽管内层 PUL 具有多孔性，但外层 BC 呈现纳米纤维形态，作为阻止微生物入侵的屏障。由于 PUL 侧的水凝胶特性，BC/PUL 的平衡含水量高于 85%，适合吸收伤口床中的渗出物。在模拟伤口液中，PUL 层在 14 天内失去了超过 90%的重量，在溶菌酶溶液中失去了超过 99%的重量，介导了 VtC 和 VtE 的共同释放。薄的 BC 侧具有足够的强度（⁓22 MPa）和应变（>30%），以承受伤口表面弯曲产生的拉伸应力而不会破裂，而厚的 PUL 侧具有柔韧性（>70%应变），可以在压缩应力下适应伤口床而不会造成伤害。使用 L929 成纤维细胞进行的体外研究表明 PUL 侧具有抗粘连和可移除性。观察到 VtC 和 VtE 在抗氧化活性、伤口闭合和胶原合成方面的协同作用。因此，BC/PUL-VtC/VtE 有望成为廉价且环保的临时伤口敷料。
