Dong Qinyong, Li Dong, Wu Yangliu, Zhou Chunran, Lin Yongxi, Miao Peijuan, Li Jiaqi, Pan Canping
Innovation Center of Pesticide Research, Department of Applied Chemistry, College of Science, China Agricultural University, Beijing, 100193, China.
Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, China.
J Plant Physiol. 2023 Oct;289:154095. doi: 10.1016/j.jplph.2023.154095. Epub 2023 Sep 16.
Few studies have been conducted to investigate the impact of pesticides on the secondary metabolism of traditional Chinese medicine and strategies to mitigate the toxicity of pesticide-induced oxidative stress. The current study focuses on evaluating the potential impacts of nano selenium (NSe) and imidacloprid (IMI) on the quality, physiological biochemistry, and secondary metabolites in Perilla frutescens (L.) Britt. (P. frutescens). The study utilized metabolome analysis to explore the toxicity mechanism of IMI. The study noted that IMI-induced stress could emerge with detrimental effects by targeting the destruction of the phenylpropanoid biosynthesis pathway. IMI-induced phenylpropanoid metabolism disorder resulted in an 8%, 17%, 25%, 10%, 65%, and 29% reduction in phenylalanine, coniferyl aldehyde, ferulic acid, cafestol, p-coumaraldehyde, and p-coumaric acid levels, respectively. Under the treatment of exogenous NSe, the levels of these metabolites were increased by 16%, 32%, 22%, 22%, 92%, and 29%, respectively. The application of exogenous NSe increased the levels of these metabolites and improved the biochemical disorder and quality of P. frutescens leaves by optimizing the phenylpropanoid metabolic pathway and enhancing the antioxidant system. Overall, the results suggest that foliar application of NSe could alleviate the oxidative stress toxicity induced by IMI and improve the quality of P. frutescens.
很少有研究调查农药对中药次生代谢的影响以及减轻农药诱导的氧化应激毒性的策略。当前的研究聚焦于评估纳米硒（NSe）和吡虫啉（IMI）对紫苏（Perilla frutescens (L.) Britt.）品质、生理生化及次生代谢产物的潜在影响。该研究利用代谢组分析来探究IMI的毒性机制。研究指出，IMI诱导的应激可能通过靶向破坏苯丙烷生物合成途径而产生有害影响。IMI诱导的苯丙烷代谢紊乱导致苯丙氨酸、松柏醛、阿魏酸、咖啡醇、对香豆醛和对香豆酸水平分别降低了8%、17%、25%、10%、65%和29%。在外源NSe处理下，这些代谢产物的水平分别提高了16%、32%、22%、22%、92%和29%。外源NSe的施用通过优化苯丙烷代谢途径和增强抗氧化系统，提高了这些代谢产物的水平，改善了紫苏叶片的生化紊乱和品质。总体而言，结果表明叶面喷施NSe可减轻IMI诱导的氧化应激毒性并提高紫苏品质。
