Faculty of Science, Energy and Building Services, University of Applied Sciences Esslingen, Kanalstraße 33, 73728, Esslingen, Germany.
Appl Microbiol Biotechnol. 2023 Dec;107(23):7043-7054. doi: 10.1007/s00253-023-12799-4. Epub 2023 Sep 23.
Vibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has high potential as a production strain for biotechnological production processes or other applications in biotechnology. Culture media for V. natriegens typically contain high sodium chloride concentrations. The corresponding high chloride concentrations can lead to corrosion processes on metal surfaces in bioreactors. Here we report the development of a low-chloride chemically defined medium for V. natriegens. Sodium chloride was completely replaced by the sodium salts disodium hydrogen phosphate, disodium sulfate, and sodium citrate, while keeping the total concentration of sodium ions constant. The use of citrate prevents the occurrence of precipitates, especially of ammonium magnesium phosphate. With this defined medium, high-cell-density fed-batch cultivations in laboratory-scale bioreactors using exponential feeding yielded biomass concentrations of more than 60 g L. KEY POINTS: A defined medium for V. natriegens that only contains traces of chloride was developed Corrosion processes on metal surfaces in industrial bioreactors can thus be prevented High yields of biomass can be achieved in fed-batch cultivation with this medium.
耐盐威克斯菌是一种嗜盐细菌，是目前报道的繁殖时间最快的非致病性细菌。因此，它有很高的潜力作为生产菌株，用于生物技术生产过程或生物技术的其他应用。耐盐威克斯菌的培养基通常含有高浓度的氯化钠。相应的高氯浓度会导致生物反应器中金属表面的腐蚀过程。在这里，我们报告了一种用于耐盐威克斯菌的低氯化学成分定义培养基的开发。氯化钠完全被磷酸二氢二钠、硫酸钠和柠檬酸钠的钠盐取代，同时保持钠离子的总浓度不变。使用柠檬酸盐可防止沉淀，特别是磷酸铵镁的沉淀。使用这种定义明确的培养基，在使用指数进料的实验室规模生物反应器中进行高细胞密度分批补料培养，可获得超过 60 g/L 的生物量浓度。关键点：开发了仅含有痕量氯离子的耐盐威克斯菌定义培养基，因此可以防止工业生物反应器中金属表面的腐蚀过程，使用这种培养基可以在分批补料培养中实现高生物量产量。
