College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, Shandong Province, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, Shandong Province, China.
BMC Vet Res. 2023 Sep 23;19(1):173. doi: 10.1186/s12917-023-03688-z.
Goose astrovirus 2 (GAstV-2) causes visceral gout in goslings and has resulted in significant economic losses in the goose industry of China since its outbreak in 2017. To further investigate the distribution and localization of GAstV-2 in different tissues at different times, a monoclonal antibody (mAb)-based immunohistochemical (IHC) assay was developed to detect GAstV-2. A total of 80 1-day-old healthy goslings were inoculated with GAstV-2 via the oral (n = 40) and intramuscular routes (n = 40). GAstV-2 in the tissues of interest was detected using the established IHC assay. The results showed that positive signals were detected in most tissues at 1 day post-infection (dpi). Viral antigens were mainly distributed in the cytoplasm, and the staining intensity was higher in the renal tubular epithelial cells than in other cells. Taken together, our data demonstrated that GAstV-2 has a broad tissue tropism and primarily targets the kidneys. These results are likely to provide a scientific basis for further elucidation of the pathogenesis of GAstV-2.
鹅星状病毒 2（GAstV-2）可引起雏鹅内脏痛风，自 2017 年爆发以来，给中国养鹅业造成了巨大的经济损失。为了进一步研究 GAstV-2 在不同时间不同组织中的分布和定位，我们开发了一种基于单克隆抗体（mAb）的免疫组织化学（IHC）检测方法来检测 GAstV-2。将 80 只 1 日龄健康雏鹅通过口服（n=40）和肌肉途径（n=40）接种 GAstV-2。使用建立的 IHC 检测方法检测感兴趣的组织中的 GAstV-2。结果显示，感染后 1 天（dpi）大多数组织中均检测到阳性信号。病毒抗原主要分布在细胞质中，在肾小管上皮细胞中的染色强度高于其他细胞。综上所述，我们的数据表明 GAstV-2 具有广泛的组织嗜性，主要靶器官为肾脏。这些结果可能为进一步阐明 GAstV-2 的发病机制提供科学依据。
