School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
School of Pharmaceutical Sciences, Nanjing Tech University, Nanjing 211816, Jiangsu, China.
Int Immunopharmacol. 2023 Nov;124(Pt B):110954. doi: 10.1016/j.intimp.2023.110954. Epub 2023 Sep 22.
Alloferon-1 is an insect polypeptide that has anti-inflammatory, antitumor and antiviral activity. This study aimed to determine the effects of alloferon-1 on estrogen deficiency-induced osteoporosis and explore the associated mechanism using a murine model of ovariectomy (OVX)-induced osteoporosis. Results showed that alloferon-1 prevented ovariectomy‑induced body weight gain, bone loss and bone mineral content reduction, affected biochemical markers of bone turnover, and restored the microstructure of bone trabeculae. Moreover, alloferon-1 suppressed the expression of the ovariectomy‑mediated inflammatory cytokines in the vertebrae bone tissues, including nucleotide-binding oligomerization domain-like-receptor family pyrin domain-containing 3 (NLRP3), cysteinyl aspartate specific proteinase-1 (Caspase-1), interleukin 1β (IL-1β) and interleukin 18 (IL-18) which were determined by immunofluorescence staining and western blot. Overall, the present study provides evidence for the effectiveness of alloferon-1 against estrogen deficiency-induced osteoporosis, suggesting an alternative drug or an auxiliary modulator for the treatment of postmenopausal osteoporosis (PMOP).
阿洛福韦-1 是一种昆虫多肽，具有抗炎、抗肿瘤和抗病毒活性。本研究旨在通过去卵巢（OVX）诱导的骨质疏松症小鼠模型，确定阿洛福韦-1 对雌激素缺乏引起的骨质疏松症的影响，并探讨其相关机制。结果表明，阿洛福韦-1 可预防去卵巢引起的体重增加、骨丢失和骨矿物质含量减少，影响骨转换的生化标志物，并恢复骨小梁的微观结构。此外，阿洛福韦-1 抑制了卵巢切除介导的炎症细胞因子在椎骨组织中的表达，包括核苷酸结合寡聚化结构域样受体家族富含亮氨酸重复蛋白 3（NLRP3）、半胱天冬氨酸特异性蛋白酶-1（Caspase-1）、白细胞介素 1β（IL-1β）和白细胞介素 18（IL-18），这是通过免疫荧光染色和 Western blot 确定的。总之，本研究为阿洛福韦-1 防治雌激素缺乏性骨质疏松症提供了证据，提示其可能成为治疗绝经后骨质疏松症（PMOP）的一种替代药物或辅助调节剂。
