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通过添加醋酸钠在子实体分化阶段增强从底物中纤维素的酶促水解及吲哚 - 3 - 乙酸含量

Enhanced Enzymatic Hydrolysis of Cellulose From Substrate and Indole-3-Acetic Acid Content-During the Fruiting Body Differentiation Stage by Sodium Acetate Addition.

作者信息

Hou Li-Juan, Li Zheng-Peng, Li Chang-Tian, Lin Jin-Sheng, Ma Lin, Jiang Ning, Qu Shao-Xuan, Li Hui-Ping, Li Yu

机构信息

Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education for Jilin Agricultural University, Jilin, China.

Institute of Vegetable Crop, Jiangsu Academy of Agricultural Sciences, Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing, China.

出版信息

Front Fungal Biol. 2021 Nov 23;2:746313. doi: 10.3389/ffunb.2021.746313. eCollection 2021.

DOI:10.3389/ffunb.2021.746313
PMID:37744121
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10512216/
Abstract

, with high commercial, nutritional and medicinal value, is widely cultivated in tropical and subtropical regions. The effects of supplementation on mushroom yield has been studied. We showed that the optimal application of sodium acetate (NaAc) was spray application of a 0.08% concentration during the substrate mixing stage which could increase yields by up to 89.16% and enhance the enzymatic hydrolysis of cellulose and hemicellulose from the substrate. For most enzymes tested maximum activity occurred during the fruiting body growth and development stage, which led to degradation of the substrate, increasing the available nutrients for mycelial propagation and fruiting body growth and development. Meanwhile, NaAc also significantly increased the indole-3-acetic acid (IAA) content in the early fruiting body development stage of , It was observed that IAA promotes not only plant primordium differentiation; but also the primordium differentiation of edible fungi. Furthermore, treatments with three acetate salts had an increase of yield by 30.22% on average. The mechanisms by which NaAc application may improve the yield of are discussed.

摘要

具有很高的商业、营养和药用价值,在热带和亚热带地区广泛种植。已经研究了添加物对蘑菇产量的影响。我们发现,醋酸钠(NaAc)的最佳施用方法是在培养料混合阶段喷施浓度为0.08%的溶液,这可以使产量提高89.16%,并增强培养料中纤维素和半纤维素的酶解作用。对于大多数测试的酶,最大活性出现在子实体生长发育阶段,这导致培养料降解,增加了菌丝体繁殖和子实体生长发育可用的养分。同时,NaAc还显著增加了在子实体发育早期阶段的吲哚-3-乙酸(IAA)含量,据观察IAA不仅促进植物原基分化,还促进食用菌原基分化。此外,三种醋酸盐处理平均使产量提高了30.22%。讨论了施用NaAc可能提高产量的机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/65b4ee84acaa/ffunb-02-746313-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/b4b9f5f3dc03/ffunb-02-746313-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/6781922be7c2/ffunb-02-746313-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/b2dce885ac81/ffunb-02-746313-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/227816deb2fa/ffunb-02-746313-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/818a8d434ea2/ffunb-02-746313-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/ccac64c11c23/ffunb-02-746313-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/65b4ee84acaa/ffunb-02-746313-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/b4b9f5f3dc03/ffunb-02-746313-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/6781922be7c2/ffunb-02-746313-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/b2dce885ac81/ffunb-02-746313-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/227816deb2fa/ffunb-02-746313-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/818a8d434ea2/ffunb-02-746313-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/ccac64c11c23/ffunb-02-746313-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/843a/10512216/65b4ee84acaa/ffunb-02-746313-g0007.jpg

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