Liu Zengcai, Yu Ying, Hu Jingwei, Lv Ge, Zou Li
College of Forestry, Northeast Forestry University, Harbin, China.
Mycobiology. 2025 May 12;53(3):331-337. doi: 10.1080/12298093.2025.2500194. eCollection 2025.
To investigate the effects and underlying mechanisms of sodium (Na) on the growth characteristics of mycelia, a single-factor Na addition experiment was performed. We found that treatment with 10 mmol/L Na (Na10) significantly increased the growth rate (0.41 ± 0.01 cm/d) and biomass (4.27 ± 0.05 g/L) of mycelia, surpassing the control (Ck) group by 3.14% and 4.06%, respectively. In contrast, treatment with 100 mmol/L Na (Na100) resulted in a significant reduction in growth rate (0.34 ± 0.01 cm/d) and biomass (3.25 ± 0.02 g/L) of mycelia compared to the Ck group. Transcriptome analysis further revealed that low Na concentrations (10 mmol/L) promoted the accumulation of soluble sugars (7.63 ± 0.54 mg/g) and upregulated the expression of pertinent genes, thereby accelerating mycelial growth. On the other hand, high Na concentrations (100 mmol/L) led to HO accumulation (12.18 ± 0.24 μmol/g), causing toxicity in mycelia. High Na concentrations also significantly boosted the production of valuable metabolites, such as triterpenoids (19.65 ± 0.22 mg/g), although the exact mechanisms remain to be elucidated. Overall, we suggest an effective approach for accelerating mycelial growth cycles and enhancing the production of high-value bioactive compounds from .
为研究钠（Na）对菌丝体生长特性的影响及其潜在机制，进行了单因素钠添加实验。我们发现，用10 mmol/L Na（Na10）处理显著提高了菌丝体的生长速率（0.41±0.01 cm/d）和生物量（4.27±0.05 g/L），分别比对照组（Ck）高出3.14%和4.06%。相比之下，与Ck组相比，用100 mmol/L Na（Na100）处理导致菌丝体的生长速率（0.34±0.01 cm/d）和生物量（3.25±0.02 g/L）显著降低。转录组分析进一步表明，低钠浓度（10 mmol/L）促进了可溶性糖的积累（7.63±0.54 mg/g），并上调了相关基因的表达，从而加速了菌丝体生长。另一方面，高钠浓度（100 mmol/L）导致过氧化氢积累（12.18±0.24 μmol/g），对菌丝体产生毒性。高钠浓度还显著提高了三萜类化合物（19.65±0.22 mg/g）等有价值代谢产物的产量，尽管确切机制尚待阐明。总体而言，我们提出了一种加速菌丝体生长周期并提高其高价值生物活性化合物产量的有效方法。
