Peng Kai, Deng Jianping, Zou Nianli, Sun Xinran, Huang Weifeng, Li Ruichao, Yang Xiaorong
Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
Institute of Comparative Medicine, Yangzhou University, Yangzhou, Jiangsu, China.
Front Microbiol. 2023 Sep 7;14:1242369. doi: 10.3389/fmicb.2023.1242369. eCollection 2023.
The fourth mobile sulfonamide resistance gene has been discovered in many metagenomic datasets. However, there is no reports of it in cultured bacteria. In this study, a positive clinical SC2020597 was obtained by conventional isolation methods and characterized by species identification and antimicrobial susceptibility testing. Meanwhile, the genomic DNA was sequenced using both long-read and short-read methods. Following that, the complete genome was analyzed by bioinformatic methods. The gene in SC2020597 differed from the identified in metagenomic data by one amino acid and could confer full resistance to sulfamethoxazole. Genetic location analysis showed that the in SC2020597 was carried by a complex chromosomally integrated hybrid plasmid. IS-like was strongly associated with the mobilization of by core genetic context analysis. To the best of our knowledge, this is the first report of the emergence of in clinically cultured . More important, the has the potential to spread to other bacteria with the help of mobile elements.
在许多宏基因组数据集中发现了第四个移动性磺胺耐药基因。然而，在培养细菌中尚无关于它的报道。在本研究中，通过常规分离方法获得了一株阳性临床菌株SC2020597，并通过菌种鉴定和抗菌药敏试验对其进行了表征。同时，使用长读长和短读长方法对基因组DNA进行了测序。随后，通过生物信息学方法对完整基因组进行了分析。SC2020597中的该基因与宏基因组数据中鉴定出的基因有一个氨基酸的差异，并且可以赋予对磺胺甲恶唑的完全抗性。遗传定位分析表明，SC2020597中的该基因由一个复杂的染色体整合杂交质粒携带。通过核心遗传背景分析，类插入序列与该基因的移动密切相关。据我们所知，这是临床培养菌株中该基因出现的首次报道。更重要的是，该基因有可能借助移动元件传播到其他细菌。
