State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Microbiol Spectr. 2022 Feb 23;10(1):e0161721. doi: 10.1128/spectrum.01617-21. Epub 2022 Feb 9.
A total of 1,400 samples of food animals (pigs, chickens, and ducks) were collected between July and September 2019 in China to uncover the prevalence of and its potential role in the evolution of antimicrobial resistance (AMR). An isolation of was performed and pulsed-field gel electrophoresis (PFGE) was used to uncover the genetic relationship. The AMR of isolates was comprehensively characterized using broth microdilution-based antimicrobial susceptibility testing, S1-PFGE, southern hybridization, whole-genome sequencing, and in-depth bioinformatics analysis. As a result, a total of 133 isolates were obtained. These isolates could be grouped into 41 PFGE subclades, suggesting a diverse genetic relationship. The resistance phenotypes of sulfafurazole (97.74%) and tetracycline (94.74%) were the most frequently found. Of the isolates, 51.88% were extended spectrum beta-lactamase (ESBL)-positive. Forty-three different AMR genes were revealed based on 25 genome sequences harboring Briefly, (6), (3'') and (A) genes were the most frequently observed, with the highest rate being 76.00% (19/25). Three -harboring plasmids were identified after Nanopore sequencing, including pTB31P1 (IncHI2-IncHI2A, 184,652 bp), pTB44P3 (IncI2, 62,882 bp), and pTB91P1 (IncHI2-IncHI2A, 255,882 bp). Additionally, 25 isolates harboring were clustered together with other isolates from different regions and sources available in GenBank, suggesting a possible random process of transmission in . In conclusion, is widespread in food animals in China and might be an important reservoir of AMR genes, especially and facilitate the evolution of AMR. , a member of the genus Escherichia, has been reported to transmit via the food chain and cause diseases in humans. However, the prevalence of multidrug-resistant , especially -positive isolates, has rarely been reported. Here, we collected 1,400 samples from food animals in three provinces of China and obtained 133 isolates (9.5%). We found that the prevalence of isolates was diverse, with high levels of antimicrobial resistance. Among them, 18.8% isolates carried the colistin resistance gene . Thus, may facilitate the evolution of colistin resistance as a reservoir of As far as we know, the prevalence and AMR of in the food animals in this study was first reported in China. These findings increase our understanding of the role of in public health and the evolution of antibiotic resistance.
2019 年 7 月至 9 月，在中国共采集了 1400 份食用动物（猪、鸡、鸭）样本，以揭示 的流行情况及其在抗生素耐药性（AMR）进化中的潜在作用。进行了 的分离，并使用脉冲场凝胶电泳（PFGE）揭示了遗传关系。使用肉汤微量稀释法抗菌药敏试验、S1-PFGE、Southern 杂交、全基因组测序和深入的生物信息学分析全面表征 的 AMR。结果共获得 133 株 。这些分离株可分为 41 个 PFGE 亚群，表明遗传关系多样。磺胺嘧啶（97.74%）和四环素（94.74%）的耐药表型最常见。在 中，51.88%为扩展谱β-内酰胺酶（ESBL）阳性。基于 25 个含有 的基因组序列，共揭示了 43 种不同的 AMR 基因。简要地说，（6）、（3'）和（A）基因是最常观察到的，最高率为 76.00%（19/25）。经 Nanopore 测序后鉴定了 3 个携带 的质粒，包括 pTB31P1（IncHI2-IncHI2A，184652 bp）、pTB44P3（IncI2，62882 bp）和 pTB91P1（IncHI2-IncHI2A，255882 bp）。此外，25 株携带 的分离株与 GenBank 中来自不同地区和来源的其他 分离株聚集在一起，表明 在 中可能存在 传播的随机过程。总之， 在中国的食用动物中广泛存在，可能是 AMR 基因的重要储存库，特别是 和 促进了 AMR 的进化。属于埃希氏菌属的 已被报道通过食物链传播并在人类中引起疾病。然而，多药耐药 ，尤其是 阳性 的分离株的流行情况很少被报道。在这里，我们从中国三个省份的食用动物中采集了 1400 个样本，获得了 133 个 分离株（9.5%）。我们发现 分离株的流行情况多样，具有较高的抗菌药物耐药性。其中，18.8%的 分离株携带多粘菌素耐药基因 。因此， 可能作为 的储存库，促进多粘菌素耐药的进化。据我们所知，本研究中食用动物中 的流行情况和 AMR 是首次在中国报道的。这些发现增加了我们对 在公共卫生和抗生素耐药性进化中的作用的理解。
