Liu Lei, Zhang Xuedong, Fan Xue, Zhu Xiaoyun
Department of Pathology, Peking University International Hospital, Beijing 102206, P.R. China.
Department of Pathology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China.
Mol Clin Oncol. 2023 Aug 31;19(4):82. doi: 10.3892/mco.2023.2678. eCollection 2023 Oct.
This study aimed to analyze the molecular characteristics of gastric adenocarcinoma of the fundic-gland type (GAFG) and explore the possible mechanism of tumor development. Samples from 10 Chinese patients with GAFG were collected at the Peking University International Hospital and Liaocheng People's Hospital between January 2015 and March 2022. The nucleic acid sequence of Epstein Barr virus-encoded RNA (EBV-EBER) was detected by hybridization. Genetic mutation information for , , , , , , , , , , , , and was obtained by Next-Generation Sequencing, and the relevant literature was reviewed. A total of eight instances of missense mutations were detected, consisting of seven cases with mutations, two cases with mutations, and one case with a mutation. Additionally, two patients had simultaneous missense mutations in and . Nonsynonymous mutations in , , , , , , , , , or were not observed in any cases. In addition, all tumors were EBER-negative. GAFG exhibits diversity at the molecular level, and mutations are more common than mutations, mutations, and microsatellite instability. To date, no association between EBV/ and GAFG has been found.
本研究旨在分析胃底腺型胃癌（GAFG）的分子特征，并探讨肿瘤发生的可能机制。2015年1月至2022年3月期间，从北京大学国际医院和聊城市人民医院收集了10例中国GAFG患者的样本。通过杂交检测爱泼斯坦-巴尔病毒编码RNA（EBV-EBER）的核酸序列。通过二代测序获得了 、 、 、 、 、 、 、 、 、 、 、 和 的基因突变信息，并查阅了相关文献。共检测到8例错义突变，其中7例为 突变，2例为 突变，1例为 突变。此外，2例患者在 和 同时存在错义突变。在任何病例中均未观察到 、 、 、 、 、 、 、 、 或 的非同义突变。此外，所有肿瘤均为EBER阴性。GAFG在分子水平上表现出多样性， 突变比 突变、 突变和微卫星不稳定性更常见。迄今为止，尚未发现EBV/与GAFG之间存在关联。
