来自两种假单胞菌的分化冰核结构基因中的保守重复序列。
Conserved repeats in diverged ice nucleation structural genes from two species of Pseudomonas.
作者信息
Warren G, Corotto L, Wolber P
出版信息
Nucleic Acids Res. 1986 Oct 24;14(20):8047-60. doi: 10.1093/nar/14.20.8047.
Abstract
Sequence analysis shows that an ice nucleation gene (inaW) from Pseudomonas fluorescens is related to the inaZ gene of Pseudomonas syringae. The two genes have diverged by many amino acid substitutions, and have effectively randomized the third bases of homologous codons. By reference to their potential for change, it is shown that certain conserved features must have been maintained by selection pressure. In particular, their conservation of internal sequence repetition, with three orders of repeat periodicity in each gene, suggests that the pattern of repetition is significant to the gene products' function. We propose models for the structure of the gene products in which each order of periodicity would be required for the nucleation function.
摘要
序列分析表明，荧光假单胞菌的一个冰核基因（inaW）与丁香假单胞菌的inaZ基因相关。这两个基因因许多氨基酸替换而发生了分化，并有效地使同源密码子的第三个碱基随机化。通过参考它们的变化潜力，结果表明某些保守特征必定是通过选择压力得以维持的。特别是，它们内部序列重复的保守性，每个基因都有三个重复周期的顺序，这表明重复模式对基因产物的功能具有重要意义。我们提出了基因产物结构的模型，其中每个周期顺序对于成核功能都是必需的。
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