Qian Yun, Li Yu-Jiang, Fu Yi-Wei, Liu Cui-Xia, Wang Juan, Yang Bin
Department of Digestive, Taizhou People's Hospital Affiliated to Nanjing Medical University, Taizhou 225300, China.
Int J Genomics. 2023 Sep 16;2023:4354536. doi: 10.1155/2023/4354536. eCollection 2023.
DTW Domain Containing 2 (DTWD2) is a newly identified transfer RNA-uridine aminocarboxypropyltransferase. Dysregulated expression of DTWD1 has been reported in several malignancies, nevertheless, the role of DTWD2 in cancers remains completely unknown. Here, we aimed to initially investigate the expression and role of DTWD2 in colon adenocarcinoma.
We first evaluated the transcription and mRNA levels of DTWD2 using data from The Cancer Genome Atlas. Besides, we tested its mRNA and protein expression in our enrolled retrospective cohort. Univariate and multivariate analyses were conducted to assess its prognostic value. Cellular experiments and xenografts were also performed to validate the role of DTWD2 in colon cancer progression.
DTWD2 was downregulated in colon adenocarcinoma and associated with poor prognosis. Lymph node metastasis, distant metastasis, and advanced tumor stage are all characterized by lower DTWD2 levels. Furthermore, Cox regression analysis demonstrated that DTWD2 is a novel independent prognostic factor for colon cancer patients. Finally, cellular and xenograft data demonstrated that silencing DTWD2 significantly enhanced colon cancer growth.
Low expression of DTWD2 may be a potential molecular marker for poor prognosis in colon cancer.
含DTW结构域蛋白2（DTWD2）是一种新发现的转运RNA尿苷氨基羧丙基转移酶。已有报道称DTWD1在多种恶性肿瘤中表达失调，然而，DTWD2在癌症中的作用仍完全未知。在此，我们旨在初步研究DTWD2在结肠腺癌中的表达及作用。
我们首先利用癌症基因组图谱（The Cancer Genome Atlas）的数据评估DTWD2的转录水平和mRNA水平。此外，我们在纳入的回顾性队列中检测了其mRNA和蛋白表达。进行单因素和多因素分析以评估其预后价值。还进行了细胞实验和异种移植实验以验证DTWD2在结肠癌进展中的作用。
DTWD2在结肠腺癌中表达下调，并与不良预后相关。淋巴结转移、远处转移和肿瘤晚期均以DTWD2水平较低为特征。此外，Cox回归分析表明DTWD2是结肠癌患者的一个新的独立预后因素。最后，细胞和异种移植数据表明，沉默DTWD2可显著促进结肠癌生长。
DTWD2低表达可能是结肠癌预后不良的潜在分子标志物。
