Kumar Raushan, Rizvi Syed Ibrahim
Department of Biochemistry, University of Allahabad, 211002 Allahabad, India.
Indian J Clin Biochem. 2023 Oct;38(4):512-518. doi: 10.1007/s12291-022-01070-8. Epub 2022 Sep 6.
Excessive dietary fat is mainly responsible for metabolic diseases including atherosclerosis and cardiovascular disease. We have evaluated the role of Vitamin C in an experimental hyperlipidemic model of rats (male Wistar rat 12-16 months). The hyperlipidemic model of the rat was created by treatment with an atherogenic suspension: cholesterol, cholic acid, and coconut oil, for 30 days once daily, and supplemented with Vitamin C (Ascorbic acid) doses of 0.5 g/kg body weight (orally) for the 30 days once daily. Bodyweight, fasting glucose, triglyceride, cholesterol, ROS (Reactive oxygen species), MDA (Malondialdehyde), FRAP (Ferric reducing the ability of plasma), GSH (Reduced glutathione), PCO (Protein carbonyl), PON-1(Paraoxonase-1), AGE (Advanced glycation end product), PMRS (Plasma membrane reduced system), and inflammatory cytokines (TNF-α and IL-6) were estimated in blood and plasma. Our result shows that oxidative stress, and inflammatory markers, were increased in the HFD-treated group of rats. Vitamin C supplementation protected against lipidemic and, oxidative stress. We conclude that Vitamin C may be useful in maintaining cellular redox balance and protecting against lipidemic stress.
过量的膳食脂肪是包括动脉粥样硬化和心血管疾病在内的代谢性疾病的主要原因。我们评估了维生素C在大鼠实验性高脂血症模型（12 - 16个月大的雄性Wistar大鼠）中的作用。大鼠高脂血症模型通过用致动脉粥样硬化混悬液（胆固醇、胆酸和椰子油）治疗创建，每天一次，持续30天，并在这30天内每天一次口服补充0.5 g/kg体重的维生素C（抗坏血酸）。对血液和血浆中的体重、空腹血糖、甘油三酯、胆固醇、活性氧（ROS）、丙二醛（MDA）、血浆铁还原能力（FRAP）、还原型谷胱甘肽（GSH）、蛋白质羰基（PCO）、对氧磷酶-1（PON-1）、晚期糖基化终产物（AGE）、质膜还原系统（PMRS）和炎性细胞因子（TNF-α和IL-6）进行了评估。我们的结果表明，高脂饮食处理的大鼠组中氧化应激和炎症标志物增加。补充维生素C可预防血脂异常和氧化应激。我们得出结论，维生素C可能有助于维持细胞氧化还原平衡并预防血脂应激。
