Molecular Cancer Biology Group, Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Vertebrate Biology Group, Zoology Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
Biochem Genet. 2024 Jun;62(3):1827-1839. doi: 10.1007/s10528-023-10509-3. Epub 2023 Sep 25.
Covid-19 crisis did hit many socio-economic aspects in the whole world. In the scientific research, the problem is getting even worse, since most of materials and consumable are allocated to the health sector. Many research laboratories around the world have big delay in receiving their purchases to accomplish their research projects. In the developing countries, the situation is much more difficult, since most of the funding resources are directed to the Covid-19 crisis and there is a notable increase in reagents' prices. Therefore, the aim of the present study is to make a homemade reagents for RNA purification from eukaryotic cells/tissues. The homemade phenol-based RNA extraction reagents were prepared using saturated phenol pH 4.3 (adjusted by 0.5 M citrate buffer) and guanidine thiocyanate. To validate the phenol-based reagent, RNA was purified from different biological samples (cell line, tissues, and fungi) using homemade phenol-based versus a commercial one. Concentration of RNA samples extracted from the same number of cells were compared to assess the homemade preparation of phenol-based reagent. In conclusion, homemade phenol-based reagent is cost effective and comparable to the commercial one. Using homemade phenol-based, RNA extraction was successfully purified from different biological sources.
Covid-19 危机确实冲击了全球许多社会经济方面。在科学研究中，问题变得更加严重，因为大部分材料和消耗品都分配给了卫生部门。世界各地的许多研究实验室在接收购买的物资以完成研究项目方面都有很大的延迟。在发展中国家，情况更加困难，因为大部分资金资源都用于应对 Covid-19 危机，并且试剂价格显著上涨。因此，本研究旨在制备用于从真核细胞/组织中纯化 RNA 的国产试剂。使用 pH 值为 4.3 的饱和苯酚（用 0.5 M 柠檬酸盐缓冲液调节）和硫氰酸胍制备国产基于苯酚的 RNA 提取试剂。为了验证基于苯酚的试剂，使用国产基于苯酚的试剂和商业试剂从不同的生物样本（细胞系、组织和真菌）中纯化 RNA。从相同数量的细胞中提取的 RNA 样本的浓度进行比较，以评估基于苯酚的国产试剂的制备。总之，国产基于苯酚的试剂具有成本效益且可与商业试剂相媲美。使用国产基于苯酚的试剂，成功地从不同的生物来源中纯化了 RNA。
