Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Aswan University, Aswan 81528, Egypt.
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Cairo 11865, Egypt.
Microsc Microanal. 2023 Jun 9;29(3):1178-1189. doi: 10.1093/micmic/ozad026.
Wounds can be a result of surgery, an accident, or other factors. There is still a challenge to find effective topical wound-healing agents. This study aims to investigate the wound-healing activity of chemical and green synthesized chitosan nanoparticles (Ch-NPs) using Lawsonia inermis leaves extract. The nanoparticles were morphologically and chemically characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (HRTEM). Forty-five adult female albino rats were randomly divided into three groups. The cutaneous surgical wounds were topically treated with 0.9% normal saline (control group), green Ch-NPs (second group), and chemical Ch-NPs gels (third group), respectively. The clinical picture of wounds and histopathological changes were assessed on the 3rd, 7th, 14th, and 21st days post-treatment. X-ray diffraction analysis revealed great crystallinity and purity of nanoparticles. The studied nanoparticles increased the wound contraction percent (WC%), reduced healing time and wound surface area (WSA), and these results were backed up by histological findings that indicated improved epithelialization, dermal differentiation, collagen deposition, and angiogenesis in treated rats compared with control rats (p < 0.05). We concluded that the wound-healing effects of the studied nanoparticles are encouraging, and further studies for complete assessment are still needed.
伤口可能是手术、事故或其他因素造成的。目前仍然需要寻找有效的局部伤口愈合剂。本研究旨在探讨用指甲花（Lawsonia inermis）叶提取物合成的化学和绿色壳聚糖纳米粒子（Ch-NPs）的伤口愈合活性。通过 X 射线衍射（XRD）、傅里叶变换红外光谱（FTIR）和高分辨率透射电子显微镜（HRTEM）对纳米粒子进行形态和化学表征。将 45 只成年雌性白化大鼠随机分为三组。分别用 0.9%生理盐水（对照组）、绿色 Ch-NPs（第二组）和化学 Ch-NPs 凝胶（第三组）局部处理皮肤外科伤口。在治疗后第 3、7、14 和 21 天评估伤口的临床图片和组织病理学变化。X 射线衍射分析表明纳米粒子具有很大的结晶度和纯度。研究中的纳米粒子增加了伤口收缩百分比（WC%），减少了愈合时间和伤口表面积（WSA），这些结果得到组织学发现的支持，表明与对照组大鼠相比，治疗组大鼠的上皮化、真皮分化、胶原蛋白沉积和血管生成得到了改善（p < 0.05）。我们得出结论，研究中的纳米粒子的伤口愈合效果令人鼓舞，但仍需要进一步研究以进行全面评估。
