Metwally Asmaa A, Abdel-Hady Abdel-Nasser A A, Haridy Mohie A M, Ebnalwaled Khaled, Saied AbdulRahman A, Soliman Ahmed S
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Aswan University, Aswan, 81511, Egypt.
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt.
Environ Sci Pollut Res Int. 2022 Apr;29(16):23975-23987. doi: 10.1007/s11356-021-17670-5. Epub 2021 Nov 24.
Wound healing is one of the utmost medical issues in human and veterinary medicine, which explains the urgent need for developing new agents that possess wound healing activities. The present study aimed to assess the effectiveness of green and chemical zinc oxide nanoparticles (ZnO-NPs) for wound healing. ZnO-NPs (green using Lawsonia inermis leaf extract and chemical) were synthesized and characterized by X-ray powder diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, and high-resolution transmission electron microscopy (HRTEM). The gels containing the nanomaterials were prepared and inspected. Forty-five albino rats were divided into three groups, the control group was treated with normal saline 0.9%, and the other two groups were treated with gels containing green or chemical ZnO-NPs, respectively. On the 3, 7, 14, and 21 days post-treatment (PT), the wounds were clinicopathologically examined. Both nanomaterials have good crystallinity and high purity, but green ZnO-NPs have a longer nanowire length and diameter than chemical ZnO-NPs. The formed gels were highly viscous with a pH of 6.5 to 7. The treated groups with ZnO-NP gels showed clinical improvement, as decreased wound surface area (WSA) percent (WSA%), increased wound contraction percent (WC%), and reduced healing time (p < 0.05) when compared with the control group. The histological scoring showed that the epithelialization score was significantly higher at the 21 day post-treatment in the treated groups than in the control group (p < 0.05), but the vasculature, necrosis, connective tissue formation, and collagen synthesis scores were mostly similar. The green and chemical ZnO-NP gels showed promising wound healing properties; however, the L. inermis-mediated ZnO-NPs were more effective.
伤口愈合是人类医学和兽医学中极为重要的医学问题之一，这也解释了为何迫切需要研发具有伤口愈合活性的新型药物。本研究旨在评估绿色和化学合成的氧化锌纳米颗粒（ZnO-NPs）对伤口愈合的有效性。通过X射线粉末衍射（XRD）、傅里叶变换红外（FTIR）光谱和高分辨率透射电子显微镜（HRTEM）对采用无患子叶提取物绿色合成及化学合成的ZnO-NPs进行了表征。制备并检查了含有纳米材料的凝胶。将45只白化大鼠分为三组，对照组用0.9%的生理盐水治疗，另外两组分别用含有绿色或化学ZnO-NPs的凝胶治疗。在治疗后（PT）的第3、7、14和21天，对伤口进行临床病理检查。两种纳米材料均具有良好的结晶度和高纯度，但绿色ZnO-NPs的纳米线长度和直径比化学ZnO-NPs更长。所形成的凝胶具有高粘性，pH值为6.5至7。与对照组相比，用ZnO-NP凝胶治疗的组显示出临床改善，如伤口表面积（WSA）百分比降低（WSA%）、伤口收缩百分比（WC%）增加以及愈合时间缩短（p < 0.05）。组织学评分显示，治疗组在治疗后第21天的上皮化评分显著高于对照组（p < 0.05），但血管生成、坏死、结缔组织形成和胶原蛋白合成评分大多相似。绿色和化学ZnO-NP凝胶均显示出有前景的伤口愈合特性；然而，无患子介导的ZnO-NPs更有效。
