斯洛文尼亚 2022 年猴痘病毒爆发的分子流行病学研究。

Molecular epidemiology of the 2022 monkeypox virus outbreak in Slovenia.

机构信息

Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

Department of Sexually Transmitted Diseases, Dermatology Clinic, Ljubljana University Medical Center, Ljubljana, Slovenia.

出版信息

Acta Dermatovenerol Alp Pannonica Adriat. 2023 Sep;32(3):111-117.

PMID:37749971

Abstract

INTRODUCTION

Monkeypox virus (MPXV), typically endemic in West and Central Africa, has raised global concern due to the recent outbreak in several non-endemic countries with human-to-human transmission. Here we present a comprehensive analysis of MPXV genomes from Slovenia.

METHODS

Two real-time polymerase chain reaction (RT-PCR) assays for Orthopoxvirus (OPV) and MPXV genes were used for laboratory confirmation of mpox. Complete MPXV genomic sequences were obtained using nanopore long reads and Illumina technology. Phylogenetic analyses compared the Slovenian MPXV sequences with the global sequences.

RESULTS

A total of 49 laboratory-confirmed mpox cases were diagnosed in Slovenia in 2022, mainly affecting males under 40. In 48 cases, a complete genome sequence was obtained and phylogenetic analysis revealed five distinct lineages (B.1, B.1.14, B.1.2, B.1.3, and A.2.1), with B.1 and B.1.3 dominating, suggesting multiple introductions into Slovenia. Genome analysis revealed significant divergence from the reference MPXV-M5312_HM12_Rivers.

CONCLUSIONS

The genetic diversity observed in the Slovenian MPXV sequences sheds light on the complex dynamics of the 2022 mpox outbreak and highlights the need for further research to understand the impact of mutations on MPXV functional characteristics and their role in the evolution and diversification of current lineages.

摘要

简介

猴痘病毒（MPXV）通常在西非和中非流行，由于最近在几个非流行国家出现了人与人之间的传播，引起了全球关注。在这里，我们对来自斯洛文尼亚的 MPXV 基因组进行了全面分析。

方法

使用两种实时聚合酶链反应（RT-PCR）检测方法对正痘病毒（OPV）和 MPXV 基因进行检测，以对猴痘进行实验室确认。使用纳米孔长读和 Illumina 技术获得完整的 MPXV 基因组序列。系统发育分析将斯洛文尼亚的 MPXV 序列与全球序列进行了比较。

结果

2022 年，斯洛文尼亚共诊断出 49 例实验室确诊的猴痘病例，主要影响 40 岁以下的男性。在 48 例病例中获得了完整的基因组序列，系统发育分析显示存在五个不同的谱系（B.1、B.1.14、B.1.2、B.1.3 和 A.2.1），其中 B.1 和 B.1.3 占主导地位，表明有多个输入进入斯洛文尼亚。基因组分析显示与参考 MPXV-M5312_HM12_Rivers 存在显著差异。