Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, Los Angeles, California, USA.
Institute of Systems Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
J Med Virol. 2023 Jan;95(1):e28036. doi: 10.1002/jmv.28036. Epub 2022 Aug 6.
Monkeypox virus (MPXV) has generally circulated in West and Central Africa since its emergence. Recently, sporadic MPXV infections in several nonendemic countries have attracted widespread attention. Here, we conducted a systematic analysis of the recent outbreak of MPXV-2022, including its genomic annotation and molecular evolution. The phylogenetic analysis indicated that the MPXV-2022 strains belong to the same lineage of the MPXV strain isolated in 2018. However, compared with the MPXV strain in 2018, in total 46 new consensus mutations were observed in the MPXV-2022 strains, including 24 nonsynonymous mutations. By assigning mutations to 187 proteins encoded by the MPXV genome, we found that 10 proteins in the MPXV are more prone to mutation, including D2L-like, OPG023, OPG047, OPG071, OPG105, OPG109, A27L-like, OPG153, OPG188, and OPG210 proteins. In the MPXV-2022 strains, four and three nucleotide substitutions are observed in OPG105 and OPG210, respectively. Overall, our studies illustrated the genome evolution of the ongoing MPXV outbreak and pointed out novel mutations as a reference for further studies.
猴痘病毒（MPXV）自出现以来一直在西非和中非地区传播。最近，几个非流行国家的散发性 MPXV 感染引起了广泛关注。在这里，我们对 2022 年的 MPXV 暴发进行了系统分析，包括其基因组注释和分子进化。系统发育分析表明，MPXV-2022 株与 2018 年分离的 MPXV 株属于同一谱系。然而，与 2018 年的 MPXV 株相比，在 MPXV-2022 株中总共观察到 46 个新的共识突变，包括 24 个非同义突变。通过将突变分配给 MPXV 基因组编码的 187 种蛋白质，我们发现 MPXV 中有 10 种蛋白质更容易发生突变，包括 D2L 样、OPG023、OPG047、OPG071、OPG105、OPG109、A27L 样、OPG153、OPG188 和 OPG210 蛋白。在 MPXV-2022 株中，分别在 OPG105 和 OPG210 中观察到四个和三个核苷酸取代。总的来说，我们的研究说明了正在进行的 MPXV 暴发的基因组进化，并指出了新的突变作为进一步研究的参考。
