Herold Dominik, Kind Jonas, Frieß Florian, Thiele Christina M
Clemens-Schöpf-Institut für Organische Chemie und Biochemie, Technische Universität Darmstadt, 64287, Darmstadt, Germany.
Photochem Photobiol Sci. 2023 Nov;22(11):2599-2606. doi: 10.1007/s43630-023-00475-5. Epub 2023 Sep 26.
Obtaining understanding of a photochemical reaction relies on the observation, identification and quantification of the compounds involved. The photochemical properties of the individual components are of particular importance, and their determination, however, is not always trivial. This is also true for the quantitative measure on the ability to absorb light, the extinction coefficient ε if more than one species i is present and two or more species absorb light of the same wavelength. In this work, it is demonstrated how pure component spectra can be obtained with a simple combination of successive and repeated ex situ illumination, UV/Vis and NMR spectroscopy. From the complementary information accessible, the wavelength-dependent extinction coefficients of all species can be calculated yielding the pure component spectra. A comparison with published data shows excellent agreement and thus proves that this approach is highly reliable.
对光化学反应的理解依赖于对所涉及化合物的观察、鉴定和定量。各组分的光化学性质尤为重要，然而，其测定并非总是轻而易举的。对于吸收光能力的定量测量也是如此，如果存在不止一种物质i，且两种或更多种物质吸收相同波长的光，则消光系数ε的测定也不简单。在这项工作中，展示了如何通过连续和重复的非原位光照、紫外/可见光谱和核磁共振光谱的简单组合来获得纯组分光谱。根据可获取的互补信息，可以计算出所有物质的波长相关消光系数，从而得到纯组分光谱。与已发表数据的比较显示出极好的一致性，因此证明了这种方法高度可靠。
