Teikyo University Institute of Medical Mycology (TIMM), 359 Otsuka, Hachioji, Tokyo, 192-0395, Japan.
Mycopathologia. 2023 Dec;188(6):1085-1088. doi: 10.1007/s11046-023-00793-3. Epub 2023 Sep 26.
Trichphyton indotineae, a species newly designated in 2020 independent of T. interdigitale, comprises highly terbinafine (TRF)-resistant dermatophytosis that is epidemic in North India and spreding to worldwide. Some clinical isolates of T. indotineae have been resistance both TRF and azoles that might be caused the treatment failure. To detect the azole resistance strains, we developed a long amplification PCR (LA-PCR) detection method for the tandem repeat of the CYP51B (encoding sterol 14a-demethylase gene) in T. indotineae. Contrasting the drug susceptibility test results with the LA-PCR results confirmed a trend toward low susceptibility to azole antifungal agents in strains with amplifications of 9.5 kbp or greater (3 or more copies of CYP51B). Our results suggest that the method could be detected rapidly of low-susceptibility strains to azole antifungal agents.
堇毛癣菌，2020 年新命名的独立于须癣毛癣菌的物种，包含高度特比萘芬（TRF）耐药的皮肤癣菌病，在印度北部流行并传播到世界各地。堇毛癣菌的一些临床分离株对 TRF 和唑类药物都有耐药性，这可能导致治疗失败。为了检测唑类药物耐药株，我们开发了一种长扩增 PCR（LA-PCR）检测方法，用于检测堇毛癣菌 CYP51B（编码甾醇 14a-脱甲基酶基因）的串联重复。将药敏试验结果与 LA-PCR 结果进行对比，证实了在扩增 9.5kbp 或更大（3 个或更多 CYP51B 拷贝）的菌株中对唑类抗真菌药物的低敏感性趋势。我们的研究结果表明，该方法可快速检测唑类抗真菌药物低敏感性菌株。
