Zeng Yetao, Zheng Haiqian, He Cheng, Zhang Chuanxu, Zhang Hongkuan, Zheng Huaiping
Key Laboratory of Marine Biotechnology of Guangdong Province, Marine Sciences Institute, Shantou University, Shantou 515063, China; Research Center of Engineering Technology for Subtropical Mariculture of Guangdong Province, Shantou 515063, China.
Key Laboratory of Marine Biotechnology of Guangdong Province, Marine Sciences Institute, Shantou University, Shantou 515063, China; Research Center of Engineering Technology for Subtropical Mariculture of Guangdong Province, Shantou 515063, China.
Comp Biochem Physiol B Biochem Mol Biol. 2024 Jan;269:110904. doi: 10.1016/j.cbpb.2023.110904. Epub 2023 Sep 24.
Doublesex and Mab-3-related transcription factor (Dmrt) is a type of transcription factor with a zinc-finger DM structural domain, which plays a significant role in sex determination and differentiation in animals. Although Dmrt has been reported in many vertebrates and invertebrates, it has rarely been studied in bivalves. In this study, a total of three members of the Dmrt gene family were identified and characterized in Crassostrea gigas, and all these CgDmrt genes contained a conserved DM domain. Analysis of the phylogenetic tree and gene structure revealed that Dmrt genes clustered on one branch may have similar functions in bivalves. Expression profiling of CgDmrt mRNA in different tissues and stages of gonad development indicated that CgDmrt3 exhibited sexually dimorphic expression and played an important role in the development of the male gonad in C. gigas. Furthermore, analysis of CgDmrt mRNA expression between fertile triploids and sterile triploids showed that CgDmrt3 may be involved in sperm production. Collectively, the systematic analysis of the CgDmrt genes will provide potential insights into the function of these genes in gonadal development.
双性和Mab-3相关转录因子（Dmrt）是一种具有锌指DM结构域的转录因子，在动物的性别决定和分化中起着重要作用。尽管Dmrt已在许多脊椎动物和无脊椎动物中被报道，但在双壳贝类中却鲜有研究。在本研究中，共鉴定并表征了太平洋牡蛎中Dmrt基因家族的三个成员，所有这些CgDmrt基因都包含一个保守的DM结构域。系统发育树和基因结构分析表明，在双壳贝类中，聚集在一个分支上的Dmrt基因可能具有相似的功能。CgDmrt mRNA在不同组织和性腺发育阶段的表达谱表明，CgDmrt3表现出性别二态性表达，并且在太平洋牡蛎雄性性腺发育中发挥重要作用。此外，对可育三倍体和不育三倍体之间CgDmrt mRNA表达的分析表明，CgDmrt3可能参与精子产生。总的来说，对CgDmrt基因的系统分析将为这些基因在性腺发育中的功能提供潜在的见解。
