Situmorang H, Hestiantoro A, Purbadi S, Wuyung P E, Werdhani R A, Harahap A, Permadi W, Sahlan M, Hadisaputra W
Faculty of Medicine, University of Indonesia, Central Jakarta, Indonesia.
Faculty of Medicine, University of Padjadjaran, Sumedang, Indonesia.
Eur J Obstet Gynecol Reprod Biol X. 2023 Jun 7;19:100204. doi: 10.1016/j.eurox.2023.100204. eCollection 2023 Sep.
Endometriosis has a major impact on women's quality of life. The two primary pathologies are chronic inflammation and altered apoptotic activity. Sulawesi propolis has been shown to have known anti-inflammatory and pro-apoptotic properties in other diseases.
To investigate the effects of Sulawesi propolis in the rat endometriosis model.
An autologous endometriosis model was created in 60 female Wistar rats by laparotomy. Rats were divided into four groups ( = 15 in each group): control group (CG), dienogest group (DG), propolis 50 mg/kg body weight (BW)/day (P50) group, and propolis 100 mg/kg BW/day (P100) group. Each treatment group was divided into three different treatment durations ( = 5 in each treatment group): 2, 4 and 6 weeks. After treatment, laparotomy was performed to determine endometriotic tissue growth, apoptosis [caspase-3 and Bcl-2-associated X/Bcl-2 (Bax/Bcl)] and inflammation [prostaglandin-E2 (PGE2) and interleukin-1B (IL-1B)].
A significant difference was seen in endometriotic tissue growth between the P50 group and the CG, with the greatest reduction in the P50 6-week (P50-6) group, reaching 70.66% of the initial area. Highest Bax/Bcl-2 mRNA expression was shown in the P50-4 and P100-4 groups, highest caspase-3 expression was shown in the P50-2 and P50-4 groups, and lowest IL-1B expression was shown in the P50-4 group; all differed significantly from the CG. No significant difference in PGE2S mRNA was found between the groups.
Sulawesi propolis extract suppressed endometriotic tissue growth in the rat model by increasing apoptotic activity. The effects were time-dependent, with 50 mg/kg BW as the optimal dose.
子宫内膜异位症对女性生活质量有重大影响。两种主要病理变化是慢性炎症和凋亡活性改变。苏拉威西蜂胶已被证明在其他疾病中具有抗炎和促凋亡特性。
研究苏拉威西蜂胶对大鼠子宫内膜异位症模型的影响。
通过剖腹手术在60只雌性Wistar大鼠中建立自体子宫内膜异位症模型。大鼠分为四组（每组n = 15）：对照组（CG）、地诺孕素组（DG）、蜂胶50 mg/kg体重（BW）/天（P50）组和蜂胶100 mg/kg BW/天（P100）组。每个治疗组再分为三个不同的治疗持续时间（每个治疗组n = 5）：2周、4周和6周。治疗后，进行剖腹手术以确定异位内膜组织生长、凋亡[半胱天冬酶-3和Bcl-2相关X蛋白/Bcl-2（Bax/Bcl）]和炎症[前列腺素-E2（PGE2）和白细胞介素-1β（IL-1β）]情况。
P50组与CG组之间在异位内膜组织生长方面存在显著差异，P50 6周（P50-6）组减少最为明显，降至初始面积的70.66%。P50-4组和P100-4组显示出最高的Bax/Bcl-2 mRNA表达，P50-2组和P50-4组显示出最高的半胱天冬酶-3表达，P50-4组显示出最低的IL-1β表达；所有这些均与CG组有显著差异。各组之间未发现PGE2S mRNA有显著差异。
苏拉威西蜂胶提取物通过增加凋亡活性抑制大鼠模型中的异位内膜组织生长。其作用具有时间依赖性，50 mg/kg BW为最佳剂量。
