Cofrades Susana, Hernández-Martín Marina, Garcimartín Alba, Saiz Arancha, López-Oliva M Elvira, Benedí Juana, Álvarez María Dolores
Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), 28040 Madrid, Spain.
Physiology Department, Pharmacy School, Complutense University of Madrid, 28040 Madrid, Spain.
Gels. 2023 Sep 7;9(9):728. doi: 10.3390/gels9090728.
Pork lard gelled emulsions stabilized with two proteins [soy protein concentrate (SPC) or a pork rind protein extract (PRP)], both with and without added silicon (Si) from diatomaceous earth powder, were gelled by microbial transglutaminase and к-carrageenan. These gelled emulsions (GEs), intended as fat replacers, were evaluated in different aspects, including microstructure and technological properties during chilling storage. In addition, in vitro gastrointestinal digestion (GID) with an analysis of lipolysis and lipid digestibility was also evaluated. All GEs showed adequate technological properties after 28 days of chilling storage, although the SPC-stabilized GEs showed better gravitational and thermal stability (4% and ~6%, respectively) during chilling storage than the PRP-stabilized ones (8 and ~12%, respectively). PRP developed larger flocculates restricting pancreatic lipase-mediated lipolysis during intestinal digestion. The addition of Si to both GE structures protected them against disruption during in vitro digestion. Accordingly, Si appears to slow down fat digestion, as reflected by higher triacylglycerides content after GID (15 and 22% vs. 10 and 18% in GEs without Si) and could become a potential candidate for use in the development of healthier meat products.
用两种蛋白质(大豆浓缩蛋白(SPC)或猪皮蛋白提取物(PRP))稳定的猪油凝胶乳液,添加或不添加来自硅藻土粉末的硅(Si),通过微生物转谷氨酰胺酶和κ-卡拉胶进行凝胶化。这些用作脂肪替代品的凝胶乳液(GEs)在不同方面进行了评估,包括冷藏储存期间的微观结构和技术性能。此外,还评估了体外胃肠消化(GID)以及脂肪分解和脂质消化率分析。冷藏储存28天后,所有GEs均表现出足够的技术性能,但SPC稳定的GEs在冷藏储存期间的重力稳定性和热稳定性(分别约为4%和6%)优于PRP稳定的GEs(分别约为8%和12%)。PRP在肠道消化过程中形成了更大的絮凝物,限制了胰脂肪酶介导的脂肪分解。在两种GE结构中添加Si可保护它们在体外消化过程中不被破坏。因此,Si似乎减缓了脂肪消化,这在GID后较高的三酰甘油含量中得到体现(含Si的GEs中为15%和22%,不含Si的GEs中为10%和18%),并且可能成为开发更健康肉类产品的潜在候选物。