Cofrades Susana, Saiz Arancha, Pérez-Mateos Miriam, Garcimartín Alba, Redondo-Castillejo Rocío, Bocanegra Aranzazu, Benedí Juana, Álvarez María Dolores
Institute of Food Science, Technology and Nutrition (ICTAN-CSIC), 28040 Madrid, Spain.
Pharmacology, Pharmacognosy and Botany Department, Pharmacy School, Complutense University of Madrid, 28040 Madrid, Spain.
Foods. 2022 Mar 2;11(5):738. doi: 10.3390/foods11050738.
This study explores the influence of carboxymethylcelullose (CMC) and methylcelullose (MC), added by simultaneous (sim) and sequential (seq) emulsification methods, on the structure, rheological parameters and in vitro lipid digestibility of pork lard O/W emulsions stabilized by soy protein concentrate (SPC). Five emulsions (SPC, SPC/CMC-sim, SPC/CMC-seq SPC/MC-sim, SPC/MC-seq) were prepared in vitro. The presence of CMC and MC, and the stage of incorporation affected the emulsion microstructure. In the SPC emulsion, lipid droplets were entrapped by a protein layer that was thicker when MC was added, providing greater resistance against environmental stresses during gastrointestinal digestion. At 37 °C, CMC incorporation produced a structural reinforcement of the SPC emulsion, whereas MC addition did not affect the network rigidity, although a delaying effect on the crossover temperature was observed, which was more evident in SPC/MC-seq. The presence and stage of CMC and MC incorporation affected the rate and extent of lipolysis, with SPC/MC-seq presenting an inferior concentration of free fatty acids. The lower extent of lipolysis observed in SPC/MC-seq may be positive in the manufacture of animal fat products in which reduced fatty acid absorption is intended.
本研究探讨了通过同时乳化(sim)和顺序乳化(seq)方法添加的羧甲基纤维素(CMC)和甲基纤维素(MC)对大豆浓缩蛋白(SPC)稳定的猪油水包油(O/W)乳液的结构、流变学参数和体外脂质消化率的影响。体外制备了五种乳液(SPC、SPC/CMC-sim、SPC/CMC-seq、SPC/MC-sim、SPC/MC-seq)。CMC和MC的存在以及添加阶段影响了乳液的微观结构。在SPC乳液中,脂质滴被蛋白质层包裹,添加MC时蛋白质层更厚,在胃肠道消化过程中对环境压力具有更大的抵抗力。在37°C时,添加CMC使SPC乳液的结构增强,而添加MC对网络刚性没有影响,尽管观察到对交叉温度有延迟作用,这在SPC/MC-seq中更为明显。CMC和MC添加的存在及阶段影响了脂肪分解的速率和程度,SPC/MC-seq的游离脂肪酸浓度较低。在旨在减少脂肪酸吸收的动物脂肪产品制造中,SPC/MC-seq中观察到的较低脂肪分解程度可能是有利的。