Li Jing, He Keying, Xu Qian
Department of Stomatology, Huadong Hospital, Fudan University, Shanghai 200437, China.
Woundhealing (Hangzhou) Biotechnology Co., Ltd., Hangzhou 310018, China.
Gels. 2023 Sep 13;9(9):744. doi: 10.3390/gels9090744.
Combinations of different biomaterials with certain formulations may lead to improved properties and have significant potential for use in tissue regeneration applications. However, previously reported studies comparing biomaterials often suffered from inconsistent processing methods or inadequate comprehensive application research, hindering a comprehension of their efficacy in tissue engineering. This report explores the significance of screening the combination of gelatine with polysaccharide materials, specifically hyaluronic acid (HA) and carboxymethyl cellulose (CMC), using the same crosslinking method used for tissue regeneration. Hydrogel scaffolds (Gel/HA and Gel/CMC) at various concentrations were developed and characterized to assess their physiochemical properties. The results demonstrated that the hydrogels exhibited desirable mechanical properties, appropriate swelling behaviour, suitable porosity, and excellent cytocompatibility. In particular, the Gel1HA1 and Gel1CMC1 hydrogels showed remarkable cellular proliferation and aggregation. Further, we performed animal studies and explored the tissue regeneration effects of the Gel1HA1 and Gel1CMC1 hydrogels. Both hydrogels exhibited an accelerated wound closure rate and promoted vessel formation in a rodent full-thickness skin excisional model. Additionally, the subcutaneous implantation model demonstrated the induction of angiogenesis and collagen deposition within the implanted hydrogel samples. Overall, the hydrogels developed in this study demonstrated promising potential for use in the regeneration of soft tissue defects and this study emphasizes the significance of screening biomaterial combinations and formulations for tissue regeneration applications.
不同生物材料与特定配方的组合可能会带来性能的提升，并在组织再生应用中具有巨大的应用潜力。然而，先前报道的比较生物材料的研究往往存在加工方法不一致或综合应用研究不足的问题，这阻碍了人们对它们在组织工程中功效的理解。本报告探讨了使用用于组织再生的相同交联方法筛选明胶与多糖材料（特别是透明质酸（HA）和羧甲基纤维素（CMC））组合的重要性。制备了不同浓度的水凝胶支架（Gel/HA和Gel/CMC）并对其进行表征，以评估它们的物理化学性质。结果表明，这些水凝胶具有理想的机械性能、适当的溶胀行为、合适的孔隙率和优异的细胞相容性。特别是，Gel1HA1和Gel1CMC1水凝胶表现出显著的细胞增殖和聚集。此外，我们进行了动物研究，探讨了Gel1HA1和Gel1CMC1水凝胶的组织再生效果。在啮齿动物全层皮肤切除模型中，这两种水凝胶均表现出加快的伤口闭合速率并促进血管形成。此外，皮下植入模型表明植入的水凝胶样品内诱导了血管生成和胶原蛋白沉积。总体而言，本研究中开发的水凝胶在软组织缺损再生方面显示出有前景的潜力，并且本研究强调了筛选用于组织再生应用的生物材料组合和配方的重要性。
