Guo Baobei, Zhang Hong, Liu Yong, Chen Jianwen, Li Junjian
Institute of Loess Plateau, Shanxi University , Taiyuan, Shanxi, China.
Pomology Institute, Shanxi Agricultural University , Taiyuan, Shanxi, China.
Microbiol Spectr. 2023 Sep 27;11(5):e0006823. doi: 10.1128/spectrum.00068-23.
Crop microbiomes are widely recognized to play a role in crop stress resistance, but the ecological processes that shape crop microbiomes under water stress are unclear. Therefore, we investigated the bacterial communities of two oat () and two wheat () genotypes under different water stress conditions. Our results show that the microbial assemblage was determined by the crop compartment niche. Host selection pressure on the bacterial community increased progressively from soil to epiphyte to endophyte pathways, leading to a decrease in bacterial community diversity and network complexity. Source tracing shows that soil is the primary source of crop microbial communities and that bulk soil is the main potential source of crop microbiota. It filters gradually through the different compartment niches of the crop. We found that the phyla Actinobacteria, Proteobacteria, Gemmatimonadota, and Myxococcota were significantly enriched in bacterial communities associated with crop-resistance enzyme activity. Crop genotype influenced the composition of the rhizosphere soil microbial community, and the composition of the phylloplane microbial community was affected by water stress. IMPORTANCE In this paper, we investigated the assembly of the plant microbiome in response to water stress. We found that the determinant of microbiome assembly under water stress was the host type and that microbial communities were progressively filtered and enriched as they moved from soil to epiphyte to endophyte communities, with the main potential source being bulk soil. We also screened for bacterial communities that were significantly associated with crop enzyme activity. Our research provides insights into the manipulation of microbes in response to crop resistance to water stress.
作物微生物群在作物抗逆性中发挥作用已得到广泛认可，但水分胁迫下塑造作物微生物群的生态过程尚不清楚。因此，我们研究了两种燕麦（）和两种小麦（）基因型在不同水分胁迫条件下的细菌群落。我们的结果表明，微生物组合由作物区室生态位决定。宿主对细菌群落的选择压力从土壤到附生植物再到内生植物途径逐渐增加，导致细菌群落多样性和网络复杂性降低。溯源分析表明，土壤是作物微生物群落的主要来源，而大体积土壤是作物微生物群的主要潜在来源。它通过作物的不同区室生态位逐渐过滤。我们发现，放线菌门、变形菌门、芽单胞菌门和粘球菌门在与作物抗性酶活性相关的细菌群落中显著富集。作物基因型影响根际土壤微生物群落的组成，而叶表面微生物群落的组成受水分胁迫影响。重要性 在本文中，我们研究了植物微生物群对水分胁迫的组装情况。我们发现，水分胁迫下微生物群组装的决定因素是宿主类型，微生物群落从土壤到附生植物再到内生植物群落移动时会逐渐被过滤和富集，主要潜在来源是大体积土壤。我们还筛选出了与作物酶活性显著相关的细菌群落。我们的研究为应对作物对水分胁迫的抗性而操纵微生物提供了见解。
