Kamińska Marta, Jastrzębska Aleksandra, Walkowiak-Przybyło Magdalena, Walczyńska Marta, Komorowski Piotr, Walkowiak Bogdan
Department of Biophysics, Institute of Materials Science and Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-537 Lodz, Poland.
Aflofarm Farmacja Polska Sp. z o.o., 95-200 Pabianice, Poland.
J Funct Biomater. 2023 Sep 18;14(9):478. doi: 10.3390/jfb14090478.
The laser surface modification of metallic implants presents a promising alternative to other surface modification techniques. A total of four alloyed metallic biomaterials were used for this study: medical steel (AISI 316L), cobalt-chromium-molybdenum alloy (CoCrMo) and titanium alloys (Ti6Al4V and Ti6Al7Nb). Samples of metallic biomaterials after machining were subjected to polishing or laser modification in two different versions. The results of surface modification were documented using SEM imaging and roughness measurement. After modification, the samples were sterilized with dry hot air, then exposed to citrate blood, washed with PBS buffer, fixed with glutaraldehyde, sputtered with a layer of gold and imaged using SEM to enable the quantification of adhered, activated and aggregated platelets on the surface of biomaterial samples. The average total number, counted in the field of view, of adhered platelets on the surfaces of the four tested biomaterials, regardless of the type of modification, did not differ statistically significantly (66 ± 81, 67 ± 75, 61 ± 70 and 57 ± 61 for AISI 316L, CoCrMo, Ti6Al4V and Ti6Al7Nb, respectively) and the average number of platelet aggregates was statistically significantly higher ( < 0.01) on the surfaces of AISI 316L medical steel (42 ± 53) and of the CoCrMo alloy (42 ± 52) compared to the surfaces of the titanium alloys Ti6Al4V (33 ± 39) and Ti6Al7Nb (32 ± 37). Remaining blood after contact was used to assess spontaneous platelet activation and aggregation in whole blood by flow cytometry. An in-depth analysis conducted on the obtained results as a function of the type of modification indicates small but statistically significant differences in the interaction of platelets with the tested surfaces of metallic biomaterials.
金属植入物的激光表面改性是一种比其他表面改性技术更具前景的替代方法。本研究共使用了四种合金化金属生物材料：医用钢（AISI 316L）、钴铬钼合金（CoCrMo）和钛合金（Ti6Al4V和Ti6Al7Nb）。加工后的金属生物材料样品以两种不同方式进行抛光或激光改性。使用扫描电子显微镜（SEM）成像和粗糙度测量记录表面改性结果。改性后，样品用干热空气灭菌，然后暴露于柠檬酸盐血液中，用磷酸盐缓冲盐水（PBS）缓冲液冲洗，用戊二醛固定，溅射一层金，并使用SEM成像，以便对生物材料样品表面上粘附、活化和聚集的血小板进行定量分析。在视野中计数的四种测试生物材料表面上粘附血小板的平均总数，无论改性类型如何，在统计学上均无显著差异（AISI 316L、CoCrMo、Ti6Al4V和Ti6Al7Nb分别为66±81、67±75、61±70和57±61），与钛合金Ti6Al4V（33±39）和Ti6Al7Nb（32±37）的表面相比，AISI 316L医用钢（42±53）和CoCrMo合金（42±52）表面上血小板聚集体的平均数量在统计学上显著更高（P<0.01）。接触后剩余的血液用于通过流式细胞术评估全血中血小板的自发活化和聚集。根据改性类型对所得结果进行的深入分析表明，血小板与测试的金属生物材料表面之间的相互作用存在微小但在统计学上显著的差异。
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