Takahashi Akira, Takahashi Shotaro, Tsujino Tetsuhiro, Isobe Kazushige, Watanabe Taisuke, Kitamura Yutaka, Watanabe Takao, Nakata Koh, Kawase Tomoyuki
, Kawasaki, Japan.
Tokyo Plastic Dental Society, Kita-ku, Tokyo, Japan.
Int J Implant Dent. 2019 Feb 25;5(1):5. doi: 10.1186/s40729-019-0160-z.
Platelet-rich plasma (PRP) is widely used in regenerative dentistry. Furthermore, it is often applied in the pretreatment of titanium implants to improve their surface bioaffinity and initial stability. However, effects of PRP application on implant surface at cellular and molecular levels remain poorly understood. Therefore, we examined platelet adhesion on commercially pure titanium (cp-Ti) plates, with a particular focus on fibrinogen (FGN), von Willebrand factor (vWF), and fibronectin (FN), in the presence or absence of plasma components.
Citrated blood samples were obtained from six healthy male volunteers, and pure-PRP (P-PRP) and pure platelet suspensions in phosphate-buffered saline (PBS) were prepared. Platelet adhesion on cp-Ti plate surface was evaluated by phalloidin staining and tetrazolium dye assay. Distribution of FGN, vWF, FN, albumin, CD62P, and CD63 was examined by immunocytochemical analysis.
Platelets in PBS suspensions rapidly and time-dependently adhered to cp-Ti plate surface, but this adhesion was substantially disturbed by the presence of plasma components. FGN was most preferably adsorbed regardless of the presence or absence of plasma components, while vWF and FN showed greater accumulation on platelet adhesion area.
Although FGN is rapidly and abundantly adsorbed on cp-Ti plate surface, vWF and FN function as major platelet adhesion molecules in citrated blood samples. After pretreatment with P-PRP, however, platelets adhered to cp-Ti much less efficiently. Therefore, P-PRP pretreatment might not directly contribute to surface functionalization, initial stabilization, and osseointegration of machined or similar types of implants.
富血小板血浆（PRP）在再生牙科中广泛应用。此外，它还常用于钛种植体的预处理，以提高其表面生物亲和力和初始稳定性。然而，PRP应用对种植体表面在细胞和分子水平上的影响仍知之甚少。因此，我们研究了在有无血浆成分的情况下，血小板在商业纯钛（cp-Ti）板上的黏附情况，特别关注纤维蛋白原（FGN）、血管性血友病因子（vWF）和纤连蛋白（FN）。
从六名健康男性志愿者采集枸橼酸化血液样本，制备纯PRP（P-PRP）和磷酸盐缓冲盐水（PBS）中的纯血小板悬浮液。通过鬼笔环肽染色和四氮唑染料测定评估血小板在cp-Ti板表面的黏附情况。通过免疫细胞化学分析检测FGN、vWF、FN、白蛋白、CD62P和CD63的分布。
PBS悬浮液中的血小板迅速且随时间依赖性地黏附于cp-Ti板表面，但这种黏附受到血浆成分的显著干扰。无论有无血浆成分，FGN最易被吸附，而vWF和FN在血小板黏附区域积累更多。
尽管FGN迅速且大量地吸附在cp-Ti板表面，但在枸橼酸化血液样本中，vWF和FN是主要的血小板黏附分子。然而，用P-PRP预处理后，血小板黏附于cp-Ti的效率大大降低。因此，P-PRP预处理可能不会直接有助于加工或类似类型种植体的表面功能化、初始稳定和骨整合。
