中国云南省简单序列重复标记的开发及遗传多样性评估
Development of Simple Sequence Repeat Markers and Genetic Diversity Evaluation of in Yunnan Province, China.
作者信息
Wang Huiling, Yang Kuan, Huang Hongping, Guo Liwei, He Xiahong
机构信息
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China.
School of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China.
出版信息
J Fungi (Basel). 2023 Sep 19;9(9):944. doi: 10.3390/jof9090944.
Round spot is a destructive disease that limits of production in China. However, the genetic diversity of its etiological agent has yet to be studied. In this work, firstly, we developed 32 polymorphic microsatellite markers using MISA and CERVUS 3.0 and selected 14 for further analysis. Then, we studied the genetic diversity of 187 isolates collected from round spot using simple sequence repeat markers and polyacrylamide gel electrophoresis. The genetic diversity ranged from 0.813 to 0.946, with 264 alleles detected at the 14 microsatellite loci. The expected average heterozygosity was 0.897.
圆斑病是一种限制中国产量的毁灭性病害。然而，其病原菌的遗传多样性尚未得到研究。在这项工作中，首先，我们使用MISA和CERVUS 3.0开发了32个多态性微卫星标记，并选择了14个进行进一步分析。然后，我们使用简单序列重复标记和聚丙烯酰胺凝胶电泳研究了从圆斑病中收集的187个分离株的遗传多样性。遗传多样性范围为0.813至0.946，在14个微卫星位点检测到264个等位基因。预期平均杂合度为0.897。
Zotero 插件