College of Pharmaceutical Sciences, Yunnan University of Traditional Chinese Medicine, Kunming 650500, China.
School of Pharmaceutical Sciences, Shenzhen University Health Science Center, Shenzhen 518060, China.
Molecules. 2019 Jan 8;24(1):213. doi: 10.3390/molecules24010213.
Root rot of has received great attention due to its threat on the plantation and sustainable utilization of . . To suppress the root-rot disease, natural ingredients are of great importance because of their environment friendly properties. In this study, we found that the methanol extract from leaves has strong antifungal effects on the growth of and resulting into root-rot disease. Essential oil (EO) thereof was found to be the most active. GC-MS analysis revealed 58 ingredients and camphor, camphene, -caryophyllene, and germacrene D were identified as the major ingredients. Further antifungal assays showed that the main compounds exhibit various degrees of inhibition against all the fungi tested. In addition, synergistic effects between . EO and chemical fungicides were examined. Finally, in vivo experiments were conducted and disclosed that root rot could be largely inhibited by the petroleum ether extract from . , indicating that could be a good source for controlling root-rot.
由于对种植园和的可持续利用构成威胁，受到了极大关注。为了抑制根腐病，天然成分因其环保特性而非常重要。在这项研究中，我们发现来自叶片的甲醇提取物对导致根腐病的和的生长具有很强的抗真菌作用。发现其精油（EO）最具活性。GC-MS 分析显示含有 58 种成分，樟脑、莰烯、-丁香烯和大根香叶烯 D 被鉴定为主要成分。进一步的抗真菌试验表明，主要化合物对所有测试的真菌均表现出不同程度的抑制作用。此外，还研究了和化学杀菌剂之间的协同作用。最后，进行了体内实验，结果表明从叶片的石油醚提取物可以很大程度上抑制根腐病，表明可能是控制根腐病的良好来源。
