Ko Ya-Zhu, Liyanage Wasantha Kumara, Shih Huei-Chuan, Tseng Min-Nan, Shiao Meng-Shin, Chiang Yu-Chung
Department of Biological Sciences, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
Department of Agricultural Biology, Faculty of Agriculture, University of Ruhuna, Kamburupitiya 81100, Sri Lanka.
J Fungi (Basel). 2023 Sep 20;9(9):950. doi: 10.3390/jof9090950.
The genus , a member of the family Botryosphaeriaceae, is an important fungal disease genus in agriculture. However, the species survey and genetic diversity in Taiwan remain unclear. This study aimed to investigate the species associated with various fruit species to explore the cryptic species diversity, validate species delimitation, and unveil cryptic genetic diversity. Overall, six species were identified, with several new records of infection identified. Additionally, phylogenetic analyses indicated that the relations of all isolates of might be paraphyletic. They were grouped with based on Automatic Barcode Gap Discovery (ABGD), Automatic Partitioning (ASAP) and structure-based clustering analyses. These analyses did not provide conclusive evidence for as a stable species. It may be necessary to gather more information to clarify the species delineation. The multiple new records of species with high genetic diversity and differentiation revealed that the diversity of in Taiwan was underestimated in the past. We found that has the highest number of haplotypes but the lowest number of haplotype and nucleotide diversities, indicating a recent population expansion. This was supported by the significant negative Tajima's and Fu and Li's * tests. The high genetic diversity, low gene flow, and host-associated differentiation of species indicate that they might harbour powerful evolutionary potential in Taiwan. This study provided critical insights into genetic variation, host-associated differentiation, and demography of species, which would be helpful for disease management of related pathogens.
葡萄座腔菌属是葡萄座腔菌科的一个成员，是农业中一种重要的真菌病害属。然而，台湾地区该属物种的调查和遗传多样性仍不清楚。本研究旨在调查与各种果实物种相关的葡萄座腔菌属物种，以探索潜在的物种多样性，验证物种划分，并揭示潜在的遗传多样性。总体而言，鉴定出了6种葡萄座腔菌属物种，并发现了一些新的感染记录。此外，系统发育分析表明，所有葡萄座腔菌属分离株之间的关系可能是并系的。基于自动条形码间隙发现（ABGD）、自动划分（ASAP）和基于结构的聚类分析，它们与[具体物种名称未给出]归为一组。这些分析没有为[具体物种名称未给出]作为一个稳定物种提供确凿证据。可能需要收集更多信息来明确物种划分。多个具有高遗传多样性和分化的葡萄座腔菌属物种新记录表明，台湾地区过去对葡萄座腔菌属的多样性估计不足。我们发现[具体物种名称未给出]的单倍型数量最多，但单倍型和核苷酸多样性数量最低，表明其种群近期有所扩张。这得到了显著负的 Tajima's D 和 Fu and Li's * 检验的支持。葡萄座腔菌属物种的高遗传多样性、低基因流和宿主相关分化表明，它们在台湾可能具有强大的进化潜力。本研究为葡萄座腔菌属物种的遗传变异、宿主相关分化和种群统计学提供了关键见解，这将有助于相关病原菌的病害管理。
