Centre for Advanced Technologies, Adam Mickiewicz University, Uniwersytetu Poznańskiego 10, 61-614 Poznan, Poland.
Faculty of Materials Science and Engineering, Warsaw University of Technology, ul. Wołoska 141, 02-507 Warsaw, Poland.
Mar Drugs. 2023 Aug 24;21(9):463. doi: 10.3390/md21090463.
Skeletal constructs of diverse marine sponges remain to be a sustainable source of biocompatible porous biopolymer-based 3D scaffolds for tissue engineering and technology, especially structures isolated from cultivated demosponges, which belong to the Verongiida order, due to the renewability of their chitinous, fibre-containing architecture focused attention. These chitinous scaffolds have already shown excellent and promising results in biomimetics and tissue engineering with respect to their broad diversity of cells. However, the mechanical features of these constructs have been poorly studied before. For the first time, the elastic moduli characterising the chitinous samples have been determined. Moreover, nanoindentation of the selected bromotyrosine-containing as well as pigment-free chitinous scaffolds isolated from selected verongiids was used in the study for comparative purposes. It was shown that the removal of bromotyrosines from chitin scaffolds results in a reduced elastic modulus; however, their hardness was relatively unaffected.
不同海洋海绵的骨骼结构仍然是组织工程和技术中生物相容多孔生物聚合物 3D 支架的可持续来源，特别是从培养的寻常海绵中分离出来的结构，由于其含甲壳质的纤维状结构具有可再生性而受到关注。这些甲壳质支架在仿生学和组织工程方面已经表现出了极好的、有前景的结果，这与其广泛的细胞多样性有关。然而，这些结构的力学性能在之前的研究中并没有得到很好的研究。本文首次确定了表征甲壳质样品的弹性模量。此外，还对从所选寻常海绵中分离出来的含有溴酪氨酸和不含色素的甲壳质支架进行了纳米压痕实验，以便进行比较。研究表明，从甲壳质支架中去除溴酪氨酸会导致弹性模量降低，但硬度相对不受影响。
