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一种从（Aplysineidae: Verongiida）海绵中快速分离即用型 3D 几丁质支架的方法。

Express Method for Isolation of Ready-to-Use 3D Chitin Scaffolds from (Aplysineidae: Verongiida) Demosponge.

机构信息

Institute of Physical Chemistry, TU Bergakademie-Freiberg, Leipziger str. 29, 09559 Freiberg, Germany.

Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, 61131 Poznan, Poland.

出版信息

Mar Drugs. 2019 Feb 22;17(2):131. doi: 10.3390/md17020131.

DOI:10.3390/md17020131
PMID:
30813373
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6409528/
Abstract

Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. (Aplysineidae: Verongiida) is well known to contain bromotyrosines with relevant bioactivity against human and animal diseases. The aim of this study was to develop an express method for the production of naturally prefabricated 3D chitin and bromotyrosine-containing extracts simultaneously. This new method is based on microwave irradiation (MWI) together with stepwise treatment using 1% sodium hydroxide, 20% acetic acid, and 30% hydrogen peroxide. This approach, which takes up to 1 h, made it possible to isolate chitin from the tube-like skeleton of and to demonstrate the presence of this biopolymer in this sponge for the first time. Additionally, this procedure does not deacetylate chitin to chitosan and enables the recovery of ready-to-use 3D chitin scaffolds without destruction of the unique tube-like fibrous interconnected structure of the isolated biomaterial. Furthermore, these mechanically stressed fibers still have the capacity for saturation with water, methylene blue dye, crude oil, and blood, which is necessary for the application of such renewable 3D chitinous centimeter-sized scaffolds in diverse technological and biomedical fields.

摘要

海绵是许多生物技术应用中天然化合物和生物材料的宝贵来源。属于 Verongiida 目（Order Verongiida）的海洋海绵已知含有甲壳素和具有生物活性的溴酪氨酸。（Aplysineidae: Verongiida）以含有对人类和动物疾病具有相关生物活性的溴酪氨酸而闻名。本研究的目的是开发一种同时生产天然预制 3D 甲壳素和含溴酪氨酸提取物的表达方法。这种新方法基于微波辐射（MWI）以及使用 1%氢氧化钠、20%乙酸和 30%过氧化氢的分步处理。这种方法需要 1 小时，可从管状骨架中分离出甲壳素，并首次证明这种海绵中存在这种生物聚合物。此外，该方法不会将甲壳素脱乙酰化为壳聚糖，并能够回收即用型 3D 甲壳素支架，而不会破坏分离生物材料独特的管状纤维互连成的结构。此外，这些机械应力纤维仍然具有与水、亚甲蓝染料、原油和血液饱和的能力，这对于在各种技术和生物医学领域应用这种可再生的 3D 甲壳素厘米大小的支架是必要的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/2c43304f8727/marinedrugs-17-00131-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/308c77f2c86a/marinedrugs-17-00131-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/369ae9515b6e/marinedrugs-17-00131-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/271dad8bcc10/marinedrugs-17-00131-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/753987344d71/marinedrugs-17-00131-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/d0cd70fead9a/marinedrugs-17-00131-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/0fefd217a296/marinedrugs-17-00131-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/0b39d93d53c5/marinedrugs-17-00131-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/3c1703933315/marinedrugs-17-00131-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/06f33120f3fd/marinedrugs-17-00131-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/c1f233fc3fc3/marinedrugs-17-00131-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/b45f4b520453/marinedrugs-17-00131-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/1d2276fea536/marinedrugs-17-00131-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/d20fad515f27/marinedrugs-17-00131-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/2c43304f8727/marinedrugs-17-00131-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/308c77f2c86a/marinedrugs-17-00131-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/369ae9515b6e/marinedrugs-17-00131-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/271dad8bcc10/marinedrugs-17-00131-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/753987344d71/marinedrugs-17-00131-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/d0cd70fead9a/marinedrugs-17-00131-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/0fefd217a296/marinedrugs-17-00131-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/0b39d93d53c5/marinedrugs-17-00131-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/3c1703933315/marinedrugs-17-00131-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/06f33120f3fd/marinedrugs-17-00131-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/c1f233fc3fc3/marinedrugs-17-00131-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/b45f4b520453/marinedrugs-17-00131-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/1d2276fea536/marinedrugs-17-00131-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/d20fad515f27/marinedrugs-17-00131-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4590/6409528/2c43304f8727/marinedrugs-17-00131-g014.jpg

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