Soh Pamela Xing Yi, Khatkar Mehar Singh, Williamson Peter
School of Life and Environmental Sciences, Faculty of Science, The University of Sydney, Camperdown, NSW 2006, Australia.
School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2006, Australia.
Vet Sci. 2023 Sep 19;10(9):581. doi: 10.3390/vetsci10090581.
There has been considerable interest in studying cancer in dogs and its potential as a model system for humans. One area of research has been the search for genetic risk variants in canine lymphoma, which is amongst the most common canine cancers. Previous studies have focused on a limited number of breeds, but none have included Border Collies. The aims of this study were to identify relationships between Border Collie lymphoma cases through an extensive pedigree investigation and to utilise relationship information to conduct genome-wide association study (GWAS) analyses to identify risk regions associated with lymphoma. The expanded pedigree analysis included 83,000 Border Collies, with 71 identified lymphoma cases. The analysis identified affected close relatives, and a common ancestor was identified for 54 cases. For the genomic study, a GWAS was designed to incorporate lymphoma cases, putative "carriers", and controls. A case-control GWAS was also conducted as a comparison. Both analyses showed significant SNPs in regions on chromosomes 18 and 27. Putative top candidate genes from these regions included , , , , and .
人们对研究犬类癌症及其作为人类模型系统的潜力有着浓厚兴趣。其中一个研究领域是在犬类淋巴瘤（最常见的犬类癌症之一）中寻找遗传风险变异。以往的研究集中在少数几个品种上，但均未包括边境牧羊犬。本研究的目的是通过广泛的系谱调查确定边境牧羊犬淋巴瘤病例之间的关系，并利用这些关系信息进行全基因组关联研究（GWAS）分析，以确定与淋巴瘤相关的风险区域。扩展的系谱分析包括83,000只边境牧羊犬，其中71例确诊为淋巴瘤。分析确定了受影响的近亲，并为54例病例确定了一个共同祖先。对于基因组研究，设计了一项GWAS，纳入淋巴瘤病例、推定的“携带者”和对照。还进行了病例对照GWAS作为比较。两项分析均显示18号和27号染色体区域存在显著的单核苷酸多态性（SNP）。这些区域推定的顶级候选基因包括 、 、 、 和 。 （注：原文中候选基因部分未给出具体基因名称，所以此处用 表示）
