School of Materials Science and Chemical Engineering, School of Physical Science and Technology, The Research Institute of Advanced Technology, Ningbo University, Ningbo 315211, Zhejiang, PR China.
XLIM Research Institute, UMR 7252 CNRS/University of Limoges, 87060 Limoges, France.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 15;305:123445. doi: 10.1016/j.saa.2023.123445. Epub 2023 Sep 21.
Surface-enhanced Raman scattering (SERS) technique with high sensitivity, reliable specificity, and rapid recognition ability exhibits attractive promise for the effective fast-monitoring of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Herein, a novel SERS-active immunoassay kit for SARS-CoV-2 nucleocapsid (N) protein was prepared by in-situ growing gold (Au) nanowire forests (NFs) onto TiCT, which was then modified onto polymethyl methacrylate (PMMA) matrix and encapsulated into kit. It was noted that the Au nanowires with fibrous structures which vertically anchored on TiCT served as perfect channels to promote photo-induced charge transfer. The synergistic action of electromagnetic and chemical effects resulted in an enhancement factor (EF) of 1.27 × 10. Furthermore, the unreliable fluctuation of the enhanced signal was eliminated by using the intrinsic Raman signal of the flexible PMMA platform, achieving an improved correlation coefficient (R) value from 0.950 to 0.990. Moreover, the as-designed immunoassay kit with both high sensitivity and remedied quantitative ability rendered by the TiCT@Au NFs-PMMA composite exhibited a powerful performance in the practical detection of N-protein with concentration low to 5.0 × 10 mg/mL.
表面增强拉曼散射（SERS）技术具有高灵敏度、可靠的特异性和快速识别能力，为有效快速监测严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）提供了有吸引力的前景。在此，通过在 TiCT 上原位生长金（Au）纳米线森林（NFs），制备了一种用于 SARS-CoV-2 核衣壳（N）蛋白的新型 SERS 活性免疫分析试剂盒，然后将其修饰到聚甲基丙烯酸甲酯（PMMA）基质上并封装到试剂盒中。值得注意的是，垂直固定在 TiCT 上的具有纤维结构的 Au 纳米线作为理想的通道，促进光致电荷转移。电磁和化学效应的协同作用导致增强因子（EF）达到 1.27×10。此外，通过使用柔性 PMMA 平台的固有拉曼信号消除了增强信号的不可靠波动，实现了相关系数（R）值从 0.950 到 0.990 的提高。此外，TiCT@Au NFs-PMMA 复合材料设计的免疫分析试剂盒具有高灵敏度和修正的定量能力，在实际检测浓度低至 5.0×10 mg/mL 的 N 蛋白时表现出强大的性能。
