Collaborative Innovation Center for Green Chemical Manufacturing and Accurate Detection, Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, Shandong, China.
State Key Laboratory of Analytical Chemistry for Life Science, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023, China.
Talanta. 2024 Jan 15;267:125230. doi: 10.1016/j.talanta.2023.125230. Epub 2023 Sep 21.
Silver nanoparticles (Ag·NPs) show promising advantages in electrochemiluminescence (ECL) owing to their favorable optical properties and biocompatibility. However, their susceptibility to oxidation and degradation in the presence of air adversely affects ECL intensity. In this study, we employed a sandwich sensing platform using silica-coated silver nanoparticles (Ag@SiO) as a novel luminescent material and cerium dioxide (CeO) as an ECL signal quencher for sensitive neuro-specific enolase (NSE) detection. The core-shell structure protected Ag NPs within the silica (SiO) layer, enhancing their ECL luminescence properties by reducing external environmental influence and preventing Ag NPs aggregation. Amino-functionalized CeO efficiently diminished Ag@SiO ECL emission through electron transfer, resulting in a "signal-off" detection mode with high sensitivity and accuracy. The detection limit reached 1.66 fg/mL, and the detection range spanned from 100 fg/mL to 500 ng/mL, showcasing a powerful biomolecule detection strategy.
银纳米粒子（Ag·NPs）由于其良好的光学性质和生物相容性，在电致化学发光（ECL）中显示出有前景的优势。然而，它们在空气中易氧化和降解，这会对 ECL 强度产生不利影响。在这项研究中，我们采用了一种三明治传感平台，使用银纳米粒子包覆的二氧化硅（Ag@SiO）作为新型发光材料，以及二氧化铈（CeO）作为 ECL 信号猝灭剂，用于灵敏的神经特异性烯醇化酶（NSE）检测。核壳结构保护了银纳米粒子，在二氧化硅（SiO）层内，通过减少外部环境影响和防止 Ag NPs 聚集，增强了其 ECL 发光性能。氨基功能化的 CeO 通过电子转移有效地抑制了 Ag@SiO ECL 发射，从而实现了高灵敏度和准确性的“信号关闭”检测模式。检测限达到 1.66 fg/mL，检测范围从 100 fg/mL 到 500 ng/mL，展示了一种强大的生物分子检测策略。
