Study on the hematological toxicity of Cyprinus carpio exposed to water-soluble fraction of crude oil and TiO nanoparticles in the dark and ultraviolet.

This study is addressing the potential toxicity concerns of crude oil in common carp (Cyprinus carpio) with the novel use of TiO-NP for enhancing photocatalytic degradation of WSF of crude oil prepared under UV light or darkness. Blood samples were taken, and the biochemical parameters were analyzed. The levels of ALT, AST and ALP were significantly higher in fish exposed to UV-treated WSF. However, they were significantly lower in the groups exposed to UV-treated TiO-NPs and the combination of WSF and TiO-NPs. The levels of total protein, triglycerides, albumin and cholesterol were significantly lower in treatments exposed to UV-treated and dark-conditioned WSF compared to the control group, but they were significantly higher in fish exposed to UV-treated TiO-NPs and the combination of WSF and TiO-NPs compared to fish exposed under dark conditions and were not significantly different from the control group. The toxicity of UV-treated WSF was significantly higher than that of dark-conditioned WSF. The toxicity of TiO-NPs was lower in the presence of UV and was similar to the control treatment. The results of the study suggests that photocatalytic TiO-NPs and UV radiation reduce toxicity of the water-soluble fraction of crude oil on common carp.