The effect of exposure of hospital employees to patients with tuberculosis on dermal reactivity to four new tuberculins.

An early (6-8 h) erythematous response to Purified Protein Derivative and to sonicate antigens (new tuberculins) prepared from Mycobacterium tuberculosis, M. vaccae, M. scrofulaceum, and M. leprae occurred much more frequently amongst hospital employees exposed to patients with tuberculosis than amongst factory workers. Biopsies taken from the skin test sites at 48 h revealed a more intense inflammatory cell infiltrate in response to PPD and the sonicate of M. tuberculosis, but not to the antigens of the other mycobacteria, amongst the hospital employees thus indicating a degree of specificity. The early response appears to be directed towards species specific antigens, but not, apparently, to the same as those that elicit the 48 h reactions. The hospital employees also had higher peripheral blood B-cell counts and total IgG levels, suggestive of an adjuvant effect. It is postulated that the early reaction results from repeated exposure to tubercle bacilli and the possible nature of the reaction is discussed.