INRAE, ONF, BioForA, UMR 0588, 45075 Orleans, France.
Plateforme de Protéomique, Université de Bordeaux, 33405 Bordeaux, France.
Biomolecules. 2023 Sep 16;13(9):1400. doi: 10.3390/biom13091400.
The Douglas fir () is a conifer native to North America that has become increasingly popular in plantations in France due to its many advantages as timber: rapid growth, quality wood, and good adaptation to climate change. Tree genetic improvement programs require knowledge of a species' genetic structure and history and the development of genetic markers. The very slow progress in this field, for Douglas fir as well as the entire genus , can be explained using the very large size of their genomes, as well as by the presence of numerous highly repeated sequences. Proteomics, therefore, provides a powerful way to access genomic information of otherwise challenging species. Here, we present the first Douglas fir proteomes acquired using nLC-MS/MS from 12 different plant organs or tissues. We identified 3975 different proteins and quantified 3462 of them, then examined the distribution of specific proteins across plant organs/tissues and their implications in various molecular processes. As the first large proteomic study of a resinous tree species with organ-specific profiling, this short note provides an important foundation for future genomic annotations of conifers and other trees.
花旗松（Douglas fir）是原产于北美的一种针叶树，由于其作为木材的诸多优势，如生长迅速、木材质量好、适应气候变化能力强，在法国的人工林种植中越来越受欢迎。树木遗传改良计划需要了解物种的遗传结构和历史，并开发遗传标记。由于基因组非常大，以及存在大量高度重复的序列，这一领域的进展非常缓慢，花旗松和整个松属都是如此。因此，蛋白质组学为获取具有挑战性的物种的基因组信息提供了一种强大的方法。在这里，我们使用纳升液相色谱-串联质谱（nLC-MS/MS）从 12 种不同的植物器官或组织中获得了第一个花旗松蛋白质组。我们鉴定了 3975 种不同的蛋白质，并对其中的 3462 种进行了定量分析，然后研究了特定蛋白质在不同植物器官/组织中的分布及其在各种分子过程中的作用。作为首个针对具有器官特异性分析的树脂树物种的大型蛋白质组学研究，这篇简短的说明为未来对针叶树和其他树木的基因组注释提供了重要基础。
