Deb Suryyani, Azharuddin Mohammad, Ramström Sofia, Ghosh Kanjaksha, Singha Santiswarup, Romu Thobias, Patra Hirak Kumar
Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, Haringhata 741249, India.
Cambridge Display Technology Ltd., Huntingdon PE29 2XG, UK.
Bioengineering (Basel). 2023 Aug 29;10(9):1020. doi: 10.3390/bioengineering10091020.
Arterial thrombosis (AT) originates through platelet-mediated thrombus formation in the blood vessel and can lead to heart attack, stroke, and peripheral vascular diseases. Restricting the thrombus growth and its simultaneous monitoring by visualisation is an unmet clinical need for a better AT prognosis. As a proof-of-concept, we have engineered a nanoparticle-based theranostic (combined therapy and monitoring) platform that has the potential to monitor and restrain the growth of a thrombus concurrently. The theranostic nanotool is fabricated using biocompatible super-paramagnetic iron oxide nanoparticles (SPIONs) as a core module tethered with the anti-platelet agent Abciximab (ReoPro) on its surface. Our in vitro feasibility results indicate that ReoPro-conjugated SPIONS (Tx@ReoPro) can effectively prevent thrombus growth by inhibiting fibrinogen receptors (GPIIbIIIa) on the platelet surface, and simultaneously, it can also be visible through non-invasive magnetic resonance imaging (MRI) for potential reporting of the real-time thrombus status.
动脉血栓形成（AT）源于血管中血小板介导的血栓形成，可导致心脏病发作、中风和外周血管疾病。限制血栓生长并通过可视化对其进行同步监测，是改善AT预后尚未满足的临床需求。作为概念验证，我们设计了一种基于纳米颗粒的诊疗（联合治疗与监测）平台，该平台有潜力同时监测和抑制血栓生长。这种诊疗纳米工具是使用生物相容性超顺磁性氧化铁纳米颗粒（SPIONs）作为核心模块制造的，其表面连接有抗血小板药物阿昔单抗（ReoPro）。我们的体外可行性结果表明，阿昔单抗偶联的SPIONs（Tx@ReoPro）可通过抑制血小板表面的纤维蛋白原受体（GPIIbIIIa）有效预防血栓生长，同时，它还可以通过无创磁共振成像（MRI）显影，用于潜在的实时血栓状态报告。
