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使用优化的体内牙槽嵴裂模型评估用于骨再生的颗粒状骨替代物

Evaluation of a Granular Bone Substitute for Bone Regeneration Using an Optimized In Vivo Alveolar Cleft Model.

作者信息

Destrez Alban, Colin Emilien, Testelin Sylvie, Devauchelle Bernard, Dakpé Stéphanie, Naudot Marie

机构信息

UR 7516 CHIMERE, University of Picardie Jules Verne, Chemin du Thil, CS 52501, 80025 Amiens, France.

Maxillofacial Surgery Department, Amiens University Hospital, Rond-point du Pr Christian Cabrol, 80054 Amiens, France.

出版信息

Bioengineering (Basel). 2023 Sep 2;10(9):1035. doi: 10.3390/bioengineering10091035.

DOI:10.3390/bioengineering10091035
PMID:37760137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10525109/
Abstract

Alveolar cleft is a common congenital deformity that requires surgical intervention, notably using autologous bone grafts in young children. Bone substitutes, in combination with mesenchymal stem cells (MSCs), have shown promise in the repair of these defects. This study aimed to evaluate the regenerative capabilities of a granular bone substitute using an optimized alveolar cleft model. Thirty-six rats underwent a surgical procedure for the creation of a defect filled with a fragment of silicone. After 5 weeks, the silicone was removed and the biomaterial, with or without Wharton's jelly MSCs, was put into the defect, except for the control group. The rats underwent μCT scans immediately and after 4 and 8 weeks. Analyses showed a statistically significant improvement in bone regeneration in the two treatment groups compared with control at weeks 4 and 8, both for bone volume (94.64% ± 10.71% and 91.33% ± 13.30%, vs. 76.09% ± 7.99%) and mineral density (96.13% ± 24.19% and 93.01% ± 27.04%, vs. 51.64% ± 16.51%), but without having fully healed. This study validates our optimized alveolar cleft model in rats, but further work is needed to allow for the use of this granular bone substitute in the treatment of bone defects.

摘要

牙槽嵴裂是一种常见的先天性畸形，需要手术干预，尤其是对幼儿使用自体骨移植。骨替代物与间充质干细胞（MSCs）联合使用，在修复这些缺损方面已显示出前景。本研究旨在使用优化的牙槽嵴裂模型评估颗粒状骨替代物的再生能力。36只大鼠接受了手术，制造一个填充有硅酮碎片的缺损。5周后，取出硅酮，除对照组外，将生物材料（含或不含脐带华通氏胶间充质干细胞）放入缺损处。大鼠在即刻、4周和8周后进行μCT扫描。分析显示，在第4周和第8周时，两个治疗组的骨再生与对照组相比有统计学上的显著改善，无论是骨体积（分别为94.64%±10.71%和91.33%±13.30%，对照组为76.09%±7.99%）还是矿物质密度（分别为96.13%±24.19%和93.01%±27.04%，对照组为51.64%±16.51%），但均未完全愈合。本研究验证了我们在大鼠中优化的牙槽嵴裂模型，但需要进一步开展工作，以便将这种颗粒状骨替代物用于治疗骨缺损。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c8f332763083/bioengineering-10-01035-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c49fdb3809e8/bioengineering-10-01035-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/e7162cda6170/bioengineering-10-01035-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/3293fc773621/bioengineering-10-01035-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/32fe5e2f37b3/bioengineering-10-01035-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/e6d26ed13a1a/bioengineering-10-01035-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c2d3566125f1/bioengineering-10-01035-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/ec7a0ec33dac/bioengineering-10-01035-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/8c580fa6e212/bioengineering-10-01035-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c8f332763083/bioengineering-10-01035-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c49fdb3809e8/bioengineering-10-01035-g0A1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/e7162cda6170/bioengineering-10-01035-g0A2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/3293fc773621/bioengineering-10-01035-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/32fe5e2f37b3/bioengineering-10-01035-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/e6d26ed13a1a/bioengineering-10-01035-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c2d3566125f1/bioengineering-10-01035-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/ec7a0ec33dac/bioengineering-10-01035-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/8c580fa6e212/bioengineering-10-01035-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0111/10525109/c8f332763083/bioengineering-10-01035-g007.jpg

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Alveolar Bone Remodeling with or without Collagen Filling of the Extraction Socket: A High-Resolution X-ray Tomography Animal Study.
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