Yuanzheng Chen, Yan Gao, Ting Li, Yanjie Fu, Peng Wu, Nan Bai
Linyi, Dongguan, and Huizhou, People's Republic of China From the Departments of Burns Surgery and Plastic Surgery, Linyi People's Hospital; the Department of Medical Cosmetology, Dongguan People's Hospital; and the Department of Burns Surgery, Huizhou Municipal Central Hospital.
Plast Reconstr Surg. 2015 May;135(5):1405-1412. doi: 10.1097/PRS.0000000000001166.
Autologous bone graft has been regarded as the criterion standard for the repair of alveolar cleft. However, the most prominent issue in alveolar cleft treatment is the high absorption rate of the bone graft. The authors' objective was to investigate the effects of an autologous iliac bone, bone marrow-derived mesenchymal stem cell, and platelet-rich fibrin mixture on the repair of dog alveolar cleft.
Twenty beagle dogs with unilateral alveolar clefts created by surgery were divided randomly into four groups: group A underwent repair with an autologous iliac bone, bone marrow-derived mesenchymal stem cell, and platelet-rich fibrin mixture; group B underwent repair with autologous iliac bone and bone marrow-derived mesenchymal stem cells; group C underwent repair with autologous iliac bone and platelet-rich fibrin; and group D underwent repair with autologous iliac bone as the control. One day and 6 months after transplantation, the transplant volumes and bone mineral density were assessed by quantitative computed tomography. All of the transplants were harvested for hematoxylin and eosin staining 6 months later.
Bone marrow-derived mesenchymal stem cells and platelet-rich fibrin transplants formed the greatest amounts of new bone among the four groups. The new bone formed an extensive union with the underlying maxilla in groups A, B, and C. Transplants with the bone marrow-derived mesenchymal stem cells, platelet-rich fibrin, and their mixture retained the majority of their initial volume, whereas the transplants in the control group showed the highest absorption rate. Bone mineral density of transplants with the bone marrow-derived mesenchymal stem cells, platelet-rich fibrin, and their mixture 6 months later was significantly higher than in the control group (p < 0.05), and was the highest in bone marrow-derived mesenchymal stem cells and platelet-rich fibrin mixed transplants. Hematoxylin and eosin staining showed that the structure of new bones formed the best in group A.
Both bone marrow-derived mesenchymal stem cells and platelet-rich fibrin are capable of improving the repair of dog alveolar cleft, and the mixture of them is more potent than each one of them used singly for enhancing new bone regeneration.
自体骨移植一直被视为牙槽嵴裂修复的标准术式。然而，牙槽嵴裂治疗中最突出的问题是骨移植的高吸收率。作者的目的是研究自体髂骨、骨髓间充质干细胞和富血小板纤维蛋白混合物对犬牙槽嵴裂修复的影响。
通过手术制造单侧牙槽嵴裂的20只比格犬随机分为四组：A组采用自体髂骨、骨髓间充质干细胞和富血小板纤维蛋白混合物进行修复；B组采用自体髂骨和骨髓间充质干细胞进行修复；C组采用自体髂骨和富血小板纤维蛋白进行修复；D组采用自体髂骨作为对照。移植后1天和6个月，通过定量计算机断层扫描评估移植体积和骨密度。6个月后，所有移植物均取材进行苏木精-伊红染色。
骨髓间充质干细胞和富血小板纤维蛋白移植物在四组中形成的新骨量最多。A、B和C组中形成的新骨与下方的上颌骨形成广泛连接。含有骨髓间充质干细胞、富血小板纤维蛋白及其混合物的移植物保留了大部分初始体积，而对照组的移植物吸收率最高。6个月后，含有骨髓间充质干细胞、富血小板纤维蛋白及其混合物的移植物的骨密度显著高于对照组（p < 0.05），且在骨髓间充质干细胞和富血小板纤维蛋白混合移植物中最高。苏木精-伊红染色显示A组新骨结构形成最佳。
骨髓间充质干细胞和富血小板纤维蛋白均能改善犬牙槽嵴裂的修复，且二者混合物在促进新骨再生方面比单独使用任何一种更有效。
